Protein quantitative kit is based on Coomassie Brilliant Blue, the most commonly used protein concentration detection method in the world.
G-250 (Comas
smart
blue
G-250) method, the determination of protein concentration is fast, stable and highly sensitive. Its principle is Coomassie brilliant blue.
G-250
Combined with protein under acidic conditions, the maximum absorption peak of the dye is from
465 nm
Change to 595nm, in a certain linear range, the reaction solution
595 nm
The change of absorbance is proportional to the amount of reactive protein, which is certain.
595 nm
With the increase of absorbance, protein can be quantified. The kit contains prepared Coomassie brilliant blue dye solution and bovine serum albumin (BSA) solution as protein standard solution, and the determination range is as follows
50
g- 1000
Microgram/ml.