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(Bradford method) What is the name of the kit for determining the concentration of protein?
Bradford

Protein quantitative kit is based on Coomassie Brilliant Blue, the most commonly used protein concentration detection method in the world.

G-250 (Comas

smart

blue

G-250) method, the determination of protein concentration is fast, stable and highly sensitive. Its principle is Coomassie brilliant blue.

G-250

Combined with protein under acidic conditions, the maximum absorption peak of the dye is from

465 nm

Change to 595nm, in a certain linear range, the reaction solution

595 nm

The change of absorbance is proportional to the amount of reactive protein, which is certain.

595 nm

With the increase of absorbance, protein can be quantified. The kit contains prepared Coomassie brilliant blue dye solution and bovine serum albumin (BSA) solution as protein standard solution, and the determination range is as follows

50

g- 1000

Microgram/ml.