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Commonly used commercial blood plate culture media

1. Columbia agar 5 sheep blood

Uses: used to isolate fastidious bacteria and identify hemolytic properties

Introduction: Columbia medium is an isolation medium designed for microorganisms with demanding nutritional requirements. After adding sheep blood, its rich nutrients can support the growth of most microorganisms with different metabolic types.

2. Columbia CNA agar 5 sheep blood (Columbia CNA agar 5 sheep blood)

Usage: Selective medium for isolating fastidious bacteria and identifying hemolytic properties

Introduction: Columbia CNA agar is used to selectively culture the vast majority of Gram-positive bacteria encountered in clinical samples. The culture medium contains nalidixic acid and colimycin. Will inhibit the growth of most Gram-negative bacteria and Bacillus species.

3. Columbia agar 5 horse blood (Columbia agar 5 horse blood)

Usage: used to isolate fastidious bacteria and identify hemolytic characteristics

Introduction: Colombian horse blood culture medium is often used to isolate and culture microorganisms with demanding nutritional requirements.

4. Trypcase Soy Agar 5 sheep blood (Trypcase Soy Agar 5 sheep blood)

Usage: used to isolate bacteria and identify hemolytic characteristics

Introduction: TSA sheep blood culture medium is suitable for the isolation of a variety of common clinical microorganisms.

5. Chocolate agar PolyViteX

Usage: used to isolate fastidious bacteria

Introduction: add complex vitamin X ( PVX) chocolate agar is often used to isolate bacteria with high nutritional requirements, especially for the separation of Neisseria, Haemophilus, and Streptococcuspneumoniae in clinical samples. Effect. This medium is rich in factors X (hemin) provided by hemoglobin and factor V (NAD) provided by multivitamins. This medium is often used for subculture of purified strains.

6. Chocolate agar PolyViteX VCAT3 agar (Chocolate agar PolyViteX VCAT3 agar)

Uses: Selective isolation of Neisseria gonorrhoeae and Neisseria encephalitidis

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Introduction: Special antibiotics and antifungal agents added to this medium enable the selective isolation of Neisseria gonorrhoeae and Neisseria meningitidis from complex clinical samples.

7. Haemophilus Chocolate 2 agar

Use: Selective isolation and culture of Haemophilus

Introduction: Due to human clinical samples Medium often contains a large number of other types of bacteria, which makes the isolation of Haemophilus very difficult. This medium contains selective antibiotics and antimycotics, so it is suitable for isolating Haemophilus from complex samples.