The agar content in solid medium is about 1.5-2.0%, and the solid medium is called solid medium. The agar is heated to 100℃ for dissolution, cooled and solidified at 40℃ to make it solid.

Solid culture media can be divided into two types: one is made of natural solid substances, such as potato chips, bran, rice bran, bean cake powder, peanut cake powder, etc., which are often used in alcohol factories and breweries; The other is made by adding coagulants to liquids, such as agar solid inclined plane and solid flat medium commonly used in laboratories. This culture medium is widely used for the separation, identification, preservation, counting and observation of colony characteristics of microorganisms.