I can only give an example. Oh, it's about drugs If you don't understand, you can ask me or ask questions.

I will answer your question step by step.

I. Sampling

1, samples should be randomly selected. The general sampling quantity (more than 2 minimum packaging units) is 3 times of the inspection quantity.

2, the abnormal samples should be targeted sampling. When sampling, if abnormal or suspicious samples are found, the samples in doubt should be selected, but the packaging with mechanical damage and obvious breakage should not be used as samples. All drugs that can be seen as long mites, mildew, moth-eaten and deteriorated from the medicine, bottle mouth (inside the outer cover and around the bottle mouth) and appearance can be directly judged as unqualified without sampling inspection. The contents of the above-mentioned visual inspection cannot be used to judge whether the drug is qualified.

3. The samples after inspection shall be inspected in time. If there is any difficulty, it should be stored under the storage conditions specified by the variety (generally in a cool and dry place), and it is not allowed to be refrigerated or frozen to prevent the contaminated bacteria in the test sample from dying, damaging or reproducing due to improper storage conditions.

4. Before the test, the test sample should be kept in the original packaging state, and it is forbidden to open it. Unpacked samples shall not be used as test samples.

5. The sampling of the sample must be operated aseptically under purification conditions to prevent re-contamination. (bulk drugs, large quantities of drugs)

6. The inspection quantity of all dosage forms should be drawn from more than 2 packaging units (Chinese medicine honey pills and film agents, in addition to more than 2 packages, 4 pills and above samples should be drawn).

7. The inspection quantity of solid and semi-solid (viscous sample) preparations is 10g.

8. The test quantity of liquid preparation is 10 ml.

9. Unless otherwise specified, the inspection quantity of traditional Chinese medicine tablets is 30 ~ 50 cm2, and the inspection quantity of chemical tablets and biochemical tablets is 100cm2.

10, the inspection quantity of precious or micro-packaged samples may be reduced as appropriate. Unless otherwise specified, the oral solid preparation shall not be less than 3g, the liquid preparation shall not be less than 6ml, the test solution shall not be less than 3ml, and the external drug shall not be less than 5g.

Second, the development of culture medium.

1. Generally, if it is microbial limit, there are two kinds of media: nutrient agar medium for detecting bacteria and rose sodium agar medium for detecting mold and yeast.

2. In addition, if pathogenic bacteria are needed, Gram-negative bacteria usually use eosin methylene blue agar (EMB agar), Salmonella Shigella agar (SS agar), and Vibrio cholerae uses thiosulfate citrate bile salt sucrose agar (TCBS agar), and so on.

3, the last observation: usually count, mentioned upstairs. Then the observation of colony morphology, mainly including size, color, thickness, edge state and so on.

But when I saw your question upstairs, I was thinking, do you want to ask about the identification of unknown microorganisms, such as the types of microorganisms in the soil and the types of microorganisms in the air?

That is the isolation and identification of microorganisms. It's not a test.

In this case, nutrient agar medium and Martin agar medium are usually used.