From 1856 to 1902, Julian passed the thesis defense of inhaling ozone to treat pertussis in Paris Medical College, becoming the first person to obtain a doctorate by ozone. 1936, French doctor P·Aubourg first advocated the treatment of chronic colitis with ozone blowing in rectum. In the past 60 years, more than a dozen cases of ozone application in medical care have been developed. Ozone development in China began in 2000. After that, ozone medicine took root, blossomed and achieved remarkable results in China, which was inseparable from the promotion of famous experts such as Ni Jiaxiang and He Xiaofeng. Ozone can be used in surgery and internal medicine in the field of health care. In addition, ozone is also widely used in the field of gynecological treatment, and its strong oxidation can kill most pathogenic bacteria of gynecological diseases. In addition, ozone is composed of oxygen atoms, which will not have any influence on the ph value of women. Application of Agricultural Ozone in Agriculture Ozone is a colorless gas with a slight odor. When dissolved in water, it will become a strong oxidant and have a strong killing effect on living cells. Through the ozone generator, the oxygen in the air can be converted into ozone under the ionization of high-voltage electricity and high-frequency electricity, and then used for production. Using ozone generator, the author conducted an experimental demonstration of using ozone to control vegetable diseases and insect pests in greenhouse around Xi 'an, and achieved good results. 1. Advantages of ozone in pest control 1, safety, high efficiency and low cost. Ozone can be used for multiple purposes, and at the same time, it can control a variety of pests and diseases with low control cost. Compared with spraying pesticides, ozone application is more convenient, efficient and safe, which can greatly reduce the use of pesticides and avoid vegetable farmers from using highly toxic and residual pesticides, thus reducing the cost of medication. 2, no pollution. Ozone is unstable in dry air and can be quickly decomposed and reduced to oxygen, so it has no pollution and residue in plants and fruits, which is an important way to realize pollution-free vegetable production. 3. Improve quality and increase output. After the experiment, the abnormal fruit of tomato in greenhouse was obviously reduced, the yield was increased by about 20%, and the fruit was large, colorful and delicious. Second, use 1, seed treatment. Introducing ozone gas into clean water, and continuously stirring for 65438+/-00 minutes to obtain ozone solution. Soak the seeds in it for 15-20 minutes to kill viruses, germs and eggs on the surface of the seeds. 2, greenhouse pest control ① fumigation and disinfection. Before planting 10 day, ozone generator can be combined with high-temperature closed shed to apply ozone in the shed, and the application time should be no less than 2 hours. (2) Prevention and control of seedbed diseases and insect pests. First, seal the seedbed, apply pesticide every 10 square meter 1 minute, fumigate 10 minute, and then ventilate for 30 minutes. ③ Prevention and control of pests and diseases of protected vegetables after field planting. After slowly planting seedlings, ozone is continuously applied for 7- 10 minutes per mu of shed, then closed fumigation 15-20 minutes, and then ventilated for 30 minutes. In the shed free of pests and diseases, apply 1 time every 5-7 days, apply for 5 times continuously, and increase the application time for 5 minutes every 2-3 times until it reaches 25 minutes per mu. The fumigation time is also increased by 5- 10 minute every 2-3 times. Experiments show that ozone has a good control effect on tomato gray mold, leaf mold, early blight, late blight, cucumber downy mildew and blight, as well as greenhouse whitefly, leaf miner and aphid. However, it has no effect on pests and diseases in greenhouse soil, because the amount and concentration of ozone gas infiltrating into soil are too small. Iii. Precautions 1, reasonably determine the application amount and fumigation time. The ozone dosage and fumigation time in closed shed should be adjusted according to different crops and their growth periods. Generally speaking, growing crops are more adaptable to ozone than seedling crops. If the amount of ozone is too large or the fumigation time in the greenhouse is too long, the leaves and flowers of vegetables in the greenhouse will be poisoned and dried up, and in serious cases, the plants will die. With the growth of plants, the application amount and fumigation time can be gradually increased to achieve the purpose of controlling pests and diseases without harming vegetable crops. The release should be as uniform as possible, and the air nozzle should not be directly directed at the vegetables, but should be away from the vegetable plants for more than 0.8- 1 meter. After the fumigation time arrives, it should be ventilated in time, and the general ventilation time should not be less than 30 minutes. 2. Temperature and humidity control. When ozone is applied, the indoor temperature should be kept within the range of 10-30℃, and the control effect will be better under the condition of high air humidity. 3. When fumigating in the shed, people and animals should be strictly prevented from entering to avoid poisoning or other adverse reactions. ? The basic principle of ozone to remove pesticide residues is strong oxidant, and pesticides are organic compounds. Ozone disinfection water destroys the chemical bonds of organic pesticides through strong oxidation, making them lose their medicinal properties, and at the same time killing various bacteria and viruses on the surface to achieve the purpose of detoxification. The sterilizer for fruit and vegetable tableware in canteen is a special equipment developed by using the characteristics and performance of ozone. The equipment can quickly sterilize, disinfect and deodorize, and produce high-concentration ozone water in a short time to ensure the food safety in the canteen. This kind of equipment is generally realized by ozone germicidal lamp or ozone machine. 1. can effectively degrade pesticide residues in rice, vegetables and fruits and prolong the shelf life. 2. Used for tableware disinfection, air disinfection, freezer and storeroom disinfection, which can effectively kill bacteria and viruses and prevent the spread of diseases. Ozone is an allotrope of oxygen and a strong oxidant. Its pesticide reduction and sterilization effect is 1.5 times that of chlorine, and its sterilization speed is 600-3000 times faster than that of chlorine. Ozone naturally decays into oxygen at room temperature, and the decay period is 15 minutes to 25 minutes. Ozone is quickly converted into "ecological oxygen" in water, and there is no residue problem. Ozone is an efficient and quick disinfectant. Can quickly dissolve pesticide residues in a short time, so that bacteria and viruses can be quickly eliminated. Ozone not only has the functions of disinfection, sterilization, deodorization and decoloration, but also has many positive effects beneficial to human beings and environmental protection, such as changing the respiratory state of plants, activating plant cells, detoxifying and differentiating organic impurities. Ozone can effectively reduce and eliminate pesticides, fertilizers, biological hormone residues, various germs and pathogenic bacteria in food through water medium, and reduce the harm of pollution to human beings. 1) Soaking vegetables and fruits with smelly water generated by ozone machine can kill bacteria and viruses from the outside to the inside, degrade chemical fertilizers and pesticide residues, activate plant cells, and make you feel more at ease when eating fruits and vegetables with natural taste and rich nutrition. The pesticide residue can be removed by more than 95%, and the storage time is long. 2) Soaking broilers, raw meat, frozen fish and frozen shrimp with ozone water generated by ozone machine can kill harmful germs carried in the process of slaughter and transportation, degrade biological hormones, antibiotics, hormones and other substances harmful to human body absorbed in the feeding process, and also remove the fishy smell, so that you can safely eat chicken, fish, meat and eggs, and the taste is more delicious. 3) The ozone water produced by the ozone machine can bleach the dirt and dye color on the surface of clothes, sterilize and decompose impurities, reduce water pollution, and prevent skin diseases and beriberi from being stimulated by chemical detergent residues. 4) Wash rice with water to degrade pesticide and fertilizer residues, and then cook with purified water. The cooked rice is delicious and nutritious. (Don't use aluminum containers) Because ozone will eventually be reduced to oxygen and water, leaving no residue and no pollution to the environment. Ozone has strong oxidation, sterilization, easy decomposition and no residue, and has broad application prospects in removing pesticide residues, sterilization and preservation. Air cleanliness requirements of sterile drug production environment: What kind of purification and sterilization process should we choose in order to meet the above requirements? There are currently four methods of sterilization. Ozone sterilization is one of the important methods. But no matter what disinfection method is adopted, the above requirements must be met, and ozone sterilization is no exception. As a disinfection method to replace the traditional disinfection method, ozone is more demanding and easier to operate, otherwise it will be difficult to gain a foothold. The main advantages of ozone application in catering industry Ozone disinfection in catering industry is flexible, low cost, obvious effect and no side effects. 1. Ozone disinfection method: 1, washed and soaked with ozone water; 2. Using ozone gas for disinfection 2. Advantages of ozone disinfection 1, it can not only disinfect tableware, but also have the disinfection ability (for dining places and kitchen equipment) that other disinfection equipment and methods do not have, degrade pesticide residues in fruits and vegetables and harmful hormones contained in meat products, and avoid food poisoning. (1) Ozone gas can directly disinfect kitchens, restaurants, kitchenware, refrigerators, food shelves, farms and storerooms, and can also be cleaned and disinfected with ozone water; (2) Ozone gas or ozone water can be used to disinfect tableware that cannot withstand high temperature, such as plastics and colored porcelain products. (3) Washing vegetables with ozone water can degrade pesticide residues in fruits and vegetables and harmful hormones in meat products. 2. Ozone disinfection takes short time and is simple to operate. After disinfection, there is no need to clean. Disinfection 100 tableware (8- 10 for human use): (1) The routine procedure takes about 140 minutes, and the process is as follows: decontamination (15 minutes) cleaning. The process is as follows: decontamination (15 minutes) cleaning (15 minutes) disinfection and soaking (8 minutes) use (3) if disinfected with ozone water, it only takes 30 minutes. The process is as follows: decontamination (15min), cleaning, disinfection and soaking (15min). 3. Ozone disinfection has no harmful residue and no secondary pollution. Ozone is decomposed into oxygen by itself after disinfection, which has no odor and pollution, and disinfection is comprehensive and effective. 4. The use cost of ozone disinfection is low. Ozone disinfection mainly uses air as raw material, and the power consumption is much lower than that of disinfection cabinet. After ozone disinfection, tableware can be used directly without drying. The principle of ozone disinfection can be considered as an oxidation reaction. (1) Mechanism of inactivation of bacteria by ozone: Ozone always inactivates bacteria very quickly. Different from other fungicides, ozone can react with the lipid double bond of bacterial cell wall, penetrate into bacteria, act on protein and lipopolysaccharide, change the permeability of cells, and lead to bacterial death. Ozone also acts on nuclear substances in cells, such as purines and pyrimidines in nucleic acids, to destroy DNA. (2) Inactivation mechanism of ozone on virus: The effect of ozone on virus is firstly the four polypeptide chains of virus capsid protein, which damages RNA, especially the protein that forms RNA. After the bacteriophage was oxidized by ozone, it was observed by electron microscope that its epidermis was broken into many fragments, from which many ribonucleic acids were released, which interfered with its adsorption on sediments. There is no doubt about the thoroughness of ozone sterilization. After the germicidal lamp (1) is turned on, the indoor dirty air is gradually purified by the action of ozone and ultraviolet rays, so this lamp continuously supplies fresh air, and the free bacteria in the air are also killed in the process of ozone decomposition, which can prevent air-borne infectious diseases such as colds and prevent the infection of hepatitis and tuberculosis. Suitable for disinfection and sterilization of public places, vehicles and central air conditioners. (2) Deodorization and mildew prevention. Lighting this lamp in public places and bathrooms can not only deodorize, but also kill flies, mosquitoes and other larvae. In a dark and humid room, things can be prevented from getting moldy. ③ Application in hospital operating room and aseptic room. ④ Besides sterilization, food hygiene can delay food deterioration. ⑤ Water disinfection. It can kill bacteria in water and has the advantages of no permanent residue, no carcinogen and no peculiar smell in water. After the ultraviolet ozone germicidal lamp is ignited, special attention should be paid to the protection of people's eyes, and it is not suitable to irradiate the human body. In addition, some articles are not suitable for disinfection and sterilization with ultraviolet ozone germicidal lamp. Ozone-free ultraviolet germicidal lamp can be used, and the light with ozone wavelength of 185nm generated when the lamp is turned on can be filtered out with titanium-doped timely glass, so that the lamp does not generate or generates little ozone. Other uses of ozone are powerful bleaching agents, which are used to bleach flour and pulp and disinfect drinking water with ozone. Water only contains oxygen and has no special smell. It is also used for sewage treatment. Ozone can also be used to purify air, disinfect and treat industrial waste.