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What are the detection methods of veterinary drug residues in eggs?
I. Overview

Eggs are rich in cholesterol and nutrition. An egg weighs about 50g, contains 6-7g of protein and 5-6g of fat. The amino acid ratio of egg protein is very suitable for human physiological needs and is easily absorbed by human body, and the utilization rate is above 98%. It is an animal-derived food that people often eat.

In recent years, with the improvement of people's living standards, the production capacity of laying hens industry is over-capacity, the continuous progress and popularization of detection technology, food safety and drug residue problems are increasingly emerging, which have become the focus of global consumers and society.

Veterinary drug residue refers to the accumulation or residue of prototype drugs or their metabolites in livestock and poultry or products (such as eggs, dairy products, meat, etc.), including impurities related to veterinary drugs. ) after taking the medicine.

Veterinary drugs that are easy to cause excessive veterinary drug residues in animal-derived foods mainly include antibiotics, sulfonamides, furans, antiparasitics and hormones.

Second, the source of veterinary drug residues in eggs

In the process of centralized feeding of laying hens, veterinary drugs can not only be used to prevent and treat diseases, but also be used as feed additives to promote the growth of the body or improve the laying rate.

How are the veterinary drug residues in eggs formed?

1. Veterinary drugs have quality problems, such as excessive content and inconsistent ingredients;

2 illegal use of prohibited drugs, drugs explicitly eliminated by the state, drugs for human use or raw materials;

3. Non-standard overdose use, long-term use, does not meet the withdrawal period; Change the route, interval and frequency of administration;

4. Environmental pollution, such as chickens eating polluted water and feed. , leading to the accumulation of veterinary drugs in chickens.

Third, the present situation and harm of veterinary drug residues in eggs

Florfenicol belongs to chloramphenicol, enrofloxacin and ofloxacin belong to fluoroquinolones.

Generally speaking, the harm of veterinary drug residues to people is not acute toxicity, but mainly allergic reaction and allergic reaction, bacterial drug resistance, "three causes" and hormone action.

Allergies and allergic reactions are mainly caused by some antibiotics as therapeutic drugs or feed drug additives.

Bacterial resistance mainly refers to the selective inhibition of sensitive bacteria by animals after repeated exposure to certain antibiotics, so that drug-resistant strains multiply in large numbers and then spread from animal food to people, making people infected with such diseases ineffective or ineffective.

Among them, the "three causes" function means that veterinary drug residues can lead to abnormal fetus, gene mutation and cancer.

Four, the rapid detection scheme of veterinary drug residues in eggs

Wuhan Huamei Bio insists on defending food safety, developing, producing and selling nearly 300 kinds of food safety rapid detection products, such as enzyme-linked immunosorbent assay, colloidal gold assay, physical and chemical assay, immunoaffinity column, etc., and has formulated relevant rapid detection schemes for screening batch samples of veterinary drug residues in eggs in laboratory and on site.

1, standard for veterinary drug residues in eggs

2. Laboratory batch sample screening -ELISA kit series

Enzyme-linked immunosorbent assay kit-high sensitivity, short detection time, convenient operation, simple sample pretreatment, strong specificity and low detection cost, suitable for quantitative detection with enzyme-labeled instrument and batch sample screening in laboratory.

Among them, the four sample pretreatment methods of nitrofuran are four in one, and four items can be detected when processing a sample;

Fluoroquinolone cartridges have cross-reaction with various fluoroquinolones, and the cross-reaction rate is relatively uniform.

Enzyme labeling-Based on colorimetry, this instrument can be used with ELISA kit to read and analyze the experimental results of ELISA.

Visual ELISA plate graphic interface design, convenient sample information input function, unique customized software, rapid analysis of test results.

3. On-site batch sample screening-colloidal gold rapid detection card series

It is convenient to operate, suitable for non-professionals, and the pretreatment method is relatively simple, and the sample pretreatment can be completed with simple equipment, which is suitable for on-site detection.