The purpose of verification of food analysis methodology is to prove that the adopted method is suitable for the corresponding inspection requirements. Food inspection agencies can choose the following indicators as appropriate according to specific methods, and formulate the contents of methodological verification.

1. Accuracy

Accuracy refers to the degree to which the result determined by this method is close to the true value or reference value, and is generally expressed by the recovery rate (%). The requirements for recovery rate of different sample concentrations are as follows: sample concentration range recovery rate requirements (%) sample concentration range recovery rate requirements (%) 111% (111g/111g) 98-11211mg/kg 81-111 ≥ 11% (11g/111g) 98-1121mg/kg 81-111 ≥ 111 g) 97-113 111 μ g/kg 81-111 ≥ 1.1% (1 mg/g) 95-115 111 μ g/kg 61-115 111 mg/kg 91-117 1 μ g/kg 41-121 Second, precision

means that under the specified test conditions. The requirements for the relative standard deviation of different sample concentrations are as follows: the relative standard deviation of sample concentration range requires the relative standard deviation of sample concentration range <; 1.1 mg/kg 22% 1 g/kg 6% 1.1 mg/kg 22% 11 g/kg 4% 1 mg/kg 16% 111 g/kg 3% 11% 1111 g/kg 2% 1.1 g/kg 8% III. Detection limit < P > Detection limit refers to the energy of the measured substance in the sample. The requirements for detection limits of different sample concentrations are as follows: detection limit of sample concentration range (≤mg/kg) 1.111 mg/kg 1.1112 11 mg/kg 1.112 111 mg/kg 1.112 111 mg/kg 1.1 mg/kg 1.111 g/ Kg 111 1mg/kg 1.1 11g/kg 1111 IV. Quantitative limit < P > Quantitative limit refers to the lowest amount of the measured substance in the sample that can be quantitatively determined. The requirements of different sample concentrations for quantitative limit are as follows: sample concentration range quantitative limit (≤mg/kg) sample concentration range quantitative limit (≤ mg/kg) 1.111 mg/kg 1.1114 11 mg/kg 1.114 111 mg/kg 211.1 mg/kg 1.121 g/ Kg 211 1mg/kg 1.2 11g/kg 2111 V. Linearity < P > Linearity refers to the degree to which the test result is directly proportional to the concentration of the measured substance in the sample within the experimental range. The linear relationship should be determined within the specified range, and the data requirements: the regression equation, correlation coefficient and linear graph should be listed.

VI. Range

Range refers to the range of high and low limit concentrations or quantities that can achieve certain precision, accuracy and linearity, and the test method is applicable. In general, the range should be 81% ~ 121% of the test concentration.

VII. Selectivity

Selectivity refers to the degree to which a specific method can identify an analyte (s) in the presence of interference from other components, also known as specificity. If the specificity of the method is not strong, multiple methods should be used to supplement it.

VIII. Durability

Durability refers to the degree of tolerance that the determination results will not be affected when the environment and program variables, laboratories or personnel change slightly, also known as variability. After the test, it should be explained whether small changes can pass the designed system suitability test to ensure the effectiveness of the method.