1000ml shake flask, for example, contains 500-600ml of liquid. After inoculation, the Cordyceps militaris shake flask is still cultured for 48 hours, and it will be full after shaking for 48 hours. If it is not satisfied within 48 hours, it means that there is something wrong with the test tube.

The fermenter is full 48-72 hours after inoculation,

Standard:

Release a little strain into a clean empty test tube, and the strain accounts for 80% of the test tube. After standing vertically for 2 hours, the strain does not precipitate or float, but always remains suspended, indicating that the strain can be used successfully.

Store at 20-22 degrees 1-2 days, preferably use it up the same day. If you don't use it, cool it to 8- 10, and it can be stored for 7- 10 days.