English name AURANTII
prepare
Fructus Aurantii: remove impurities, wash, fully moisten, cut into thin slices, dry in the air, and sift out broken pulp. This product is an irregular arc-shaped thin slice with a length of 5cm and a width of 1.3cm. In the section, the mesocarp is brown to brownish, yellow-white to tawny, with 1 ~ 2 rows of dotted oil chambers near the outer edge, and a small amount of purple-brown pulp in some parts.
Stir-fried Fructus Aurantii with bran: Take pieces of Fructus Aurantii and stir-fry them with bran (Appendix II D) until the color becomes dark. This product is an irregular arc-shaped strip sheet with dark color and some focal spots.
Features this product is hemispherical with a diameter of 3 ~ 5 cm. The exocarp is brown or brown, with granular protrusions and concave oil chambers at the top of the protrusions; There are obvious traces of style residue or fruit stalk. On the profile, the peel is yellow-white, smooth and slightly raised, with a thickness of 0.4 ~ 1.3 cm. The margin is scattered in the 1 ~ 2 oil drainage cavity, and there are 7 ~ 12 valves in the medullary sac, with a few to 15 valves. The juice sac is brown to brown and contains seeds. Hard and not easy to break. It is fragrant, bitter and slightly sour.
distinguish
(1) The powder of this product is yellowish white or brownish yellow. Mesocarp cells are round or irregular, and most walls are unevenly thickened. The surface of epidermal cells in pericarp is polygonal, square or rectangular, and the pores are nearly annular, with a diameter of 16 ~ 34 microns and 5 ~ 9 accessory cells. Side view of stratum corneum. The juice sac tissue is yellowish or colorless, membranous, and the epidermal cells on the surface are long and narrow, shrinking and interlaced with the lower cells. There are square crystals of calcium oxalate in pericarp and juice sac cells, which are rhombic, polyhedral or biconical, with a diameter of 3 ~ 30 μ m. The threads, reticular ducts and tracheids are very small.
(2) Take 0.5g of this product powder, add 65438 00 ml of methanol, heat and reflux for 65438 00 minutes, filter, take 65438 00 ml of filtrate, add about 5mg of potassium tetrahydroborate, shake well, add a few drops of hydrochloric acid, and the solution is cherry red to purple red. According to high performance liquid chromatography (appendix ⅵ d).
Octadecylsilane bonded silica gel is used as a filler for chromatographic conditions and system suitability test. Acetonitrile-water (2 1.5:80.5) (pH adjusted to 3 with phosphoric acid) as mobile phase; The detection wavelength is 283 nm. The theoretical plate number should be no less than 2000 calculated by naringin peak.
Preparation of reference solution Accurately weigh the naringin reference substance 15mg dried under reduced pressure to constant weight, put it in a 50ml volumetric flask, add methanol to the scale, shake it evenly, accurately measure 5ml, put it in a 25ml volumetric flask, add methanol to the scale, shake it evenly, and get the finished product (60μg of naringin per 1ml).
To prepare the test solution, take about 0.2g of coarse powder of this product, weigh it accurately, put it in a conical flask with a stopper, add 50ml of methanol accurately, plug it, weigh it, heat and reflux it for 65438±0.5h, let it cool, plug it, weigh it again, make up the weight loss with methanol, shake it evenly, filter it, accurately measure 65438±00ml of continuous filtrate, and evaporate it. Inner diameter 1.8cm, packed in column by wet method), eluting with 25ml water, discarding aqueous solution, eluting with 50ml methanol, collecting eluate, transferring to 50ml volumetric flask, adding methanol to scale, shaking well, filtering with 0.45μm microporous membrane, and collecting filtrate.
The determination method accurately absorbs 20μl of the control solution and the test solution, and injects them into the liquid chromatograph for determination.
I see. (C27H32O 14) shall not be less than 4.0%.
Bitter, pungent, sour and warm in nature. Spleen and stomach meridian.
Function: regulating qi, regulating middle energizer, stagnation and stopping inflation. Used for chest and hypochondriac qi stagnation, fullness and pain, dyspepsia and phlegm; Gastroptosis, rectocele, genital prolapse.
Usage and dosage: 3 ~ 9g.
Pay attention to pregnant women with caution.
Store in a cool and dry place to prevent moths.
Remarks (1) Cultivars mainly include' Huangpi' and' Fructus Aurantii Immaturus'. Dai Dai', Zhu Luan Zhi' Chu Luan', Tang Cheng Zhi'.