The mushroom strains of Amanita muscaria and Agaricus blazei are divided into first grade and second grade, and the first grade is also called mother seed or test tube seed. The test tube seed is also divided into successor tube and production tube. Successor tube can be transferred to the test tube for mother seed expansion, while the production tube can only be transferred to the secondary species. The secondary seed is also called the original seed, which is expanded from the primary seed. Tertiary seeds are generally not used in the cultivation of Amanita muscaria and Agaricus blazei. A succession tube can be connected to more than 30 production tubes, and each production tube can be connected to 5-6 bottles of secondary seeds. One bottle of secondary seed can inoculate about 25 bags of cultivation bags (both ends of the bacteria), that is to say, a production tube can be inoculated with 125-130 bags of cultivation bags.