The average meat content of freshwater grouper is 78.8, the water content in the muscle is 76.00, the crude protein is 18.90, the crude fat is 3.00, and the crude ash content is 1.73. Muscle contains 18 kinds of amino acids, and the total amino acid content is 15.74 (accounting for fresh samples). Among them, the content of 8 essential amino acids for the human body is 6.88, accounting for 40.60 of the total amino acids. The content of 4 kinds of umami amino acids is 7.08, accounting for 7.08 of the total amino acids. Amount of 44.98. The essential amino acid index (EAAI) is 74.84; the branched/aromatic value of amino acids (BCAA/AAA) is 2.22. Saturated fatty acids account for 34.09% of the total fatty acid content, and unsaturated fatty acids account for 65.91% of the total fatty acid content. Among them, DHA is rich in content, accounting for 6.95% of the total fatty acid content. Rich in mineral content. The results of the comprehensive analysis show that freshwater grouper is a high-quality freshwater fish with high nutritional value.