Strain Production

The Lactobacillus pine mushrooms are similar to other mushrooms. The strains usually include mother species, original species and cultivated species. Preparation of mother seed culture medium: mother seed culture medium uses potato, glucose, and agar medium (pda). Recipe 1: 200g potatoes (peeled), 20g glucose, 20g agar, 1000m1 pine needle water;

Recipe 2: 100g corn flour, 20g glucose, 20g agar, 10o0ml pine needle water. Pine needle water must first be soaked in pine needles and boiled, and then the water can be used. Tissue separation of Lactobacillus pine mushrooms: Take fresh and tender strains picked from the wild. The strains should be in good shape and not discolored. Wrap them with roots and soil in white fine cloth or white paper. Tissue separation is performed in the inoculation box. Take a small piece of the intersection of the cap and the stem, pick it into the slope of the test tube with an inoculation needle, and gently flatten it. Since Lactobacillus pine mycorrhiza is a wild organism and fragile, there is no need to use overly irritating drugs (such as formalin) at first, and you must be careful when taking out the mushroom pieces. It is best to complete the entire process of tissue separation above the flame of an alcohol lamp.

Preparation of the original culture medium: Recipe: 78% pine sawdust, 20% rice bran or wheat bran, 1% each sugar and gypsum powder, plus a small amount of pine needle water.

Preparation of cultivated species: Formula 1: pine sawdust 35, corncob 25, loose sandy soil 18, chaff 10, wheat bran 10, compound fertilizer 2; Formula 2: pine sawdust 45, cottonseed husk 25 , pine forest soil 18, wheat bran 10, superphosphate 1, sugar and gypsum 0.5 each.

Domestication cultivation

Cultivation time is generally in summer and autumn. Although it is simple to cultivate by broadcasting in the field, some unexpected problems will occur due to the influence of climate, such as long-term drought and long-term heavy rain during the sowing period, which will cause damage to the newly entered soil bacteria and cause poor germination, but the cultivated pine trees The advantages of milk mushrooms are similar to those of wild mushrooms.

Sowing in the wild,

Pine milk mushrooms

Choose the natural growth pine forest area for more than 25 years, with a small amount of thatch growing between the woods, and draw out the "t" groove Sow the bacteria, cover the outside of the pine tree roots with mulch, grass and pine needles. After 45 days, check whether the mycelium has germinated. If it is found that there is a mycelial form in the soil layer 10-15cm away from the bacteria tank, It means that the fungus has grown. At this time, the mulch film must be removed and allowed to grow naturally. If there is a thunderstorm and the climate is suitable, mushrooms will soon appear. At the beginning, most of the buds are around the slots, and there are more clusters of mushrooms. Later, the mycelium spreads and can grow generally around the pine forest. Its growth is similar to that of wild fungi. Generally, a large number of mushrooms bloom in summer and autumn when the temperature is between 25°C and 32°C. .

There are several points that must be paid attention to during the cultivation process: First, the pine forest area must have a certain amount of scattered light, that is, 20% sunlight and 80% shade; secondly, sowing in pine forests must create a "t" trench with soil covering ; The third is that the bacterial strain is generally used once every 2 years; the fourth is to observe carefully and manage carefully. There is no need to spray drugs in the pine forest and let it grow naturally.