if you want to know the route of Tuscany, Italy, I suggest you arrange a 4-5-day trip to have enough time to explore each town and enjoy the local food and culture. The following is a possible route:

Day 1: Florence

After you arrive in Florence, you can visit the famous Uffizi Art Museum and the Holy Cross Square to enjoy the artistic and historical heritage of Florence.

The next day: Pisa and Lucca

Depart from Florence and go to Pisa to visit the famous Leaning Tower of Pisa, Cathedral and Santa Maria Cathedral. Then go to Lucca and visit the Lucca City Wall and St Martin's Church.

Day 3: Siena

Leave Florence in the morning and go to Siena to visit the city square, the Church of the Virgin Mary and the Church of Saint Catherine. In the historic center of Siena, you can enjoy bars, cafes and local cuisine.

Day 4: San Jimignano and Pienza

Depart from Siena and go to the small town of San Jimignano to enjoy the beautiful medieval buildings and castles. Next, go to Palma, where you can visit Palma Cathedral, Pianza Castle and local cheese factory.

Day 5: On the last day of Arezzo and MontePolci Yano,

You can visit churches and palaces in arezzo, and then go to MontePolci Yano, where you can taste local food and wine. There you can enjoy beautiful scenery and castles.

note: you can modify the route plan according to your interest and time, for example, add other towns or extend the travel time.