2 English reference Fritillaria pallidiflora Schrek [Landau Chinese-English Dictionary]
Fritillariae pallidiflorae, Bulbus [Xiangya Medical Dictionary]
3 Overview Fritillaria Fritillaria is the name of traditional Chinese medicine, which comes from Xinjiang Manual of Chinese Herbal Medicine [1]. Pharmacopoeia of the People's Republic of China (20 10 edition) records the pharmacopoeia standard of this Chinese patent medicine.
4 Source and place of origin Bulb of Liliaceae Fritillaria walujewii Regel in Xinjiang or F. Pallidiflora Schrenk in Yili [1]. Produced in Xinjiang [1].
5 The taste is bitter, sweet and slightly cold [1].
6 Functions are mainly used for relieving cough, resolving phlegm, clearing lung-heat and resolving hard mass [1]. Indications: cough due to lung heat, chest tightness and phlegm, tuberculosis of lymph nodes and carbuncle [1].
7 usage and dosage of Fritillaria Fritillaria: 3 ~ 9g [1].
8 Pay attention to anti-aconite and aconite [1].
9 Chemical composition This product contains imperialine [1].
10 pharmacological action This product contains imperialine, which has the effect of lowering blood pressure in animals and relieving spasm in isolated animal intestines [1].
1 1 Fritillaria Fritillaria Pharmacopoeia Standard 1 1. 1 Fritillaria Fritillaria
Yibeimu
FRITILLARIAE PALLIDIFLORAE BULBUS
1 1.2 Source This product is the dried scales of Fritillaria waLujewii Regel of Liliaceae or Fritillaria pallidi flora Schrenk of Ili Fritillaria. Excavate from May to July, remove sediment, dry in the sun, and then remove fibrous roots and skins.
1 1.3 characteristics 1 1.3. 1 Fritillaria Xinjiang is oblate and 0.5 ~1.5 cm high. The surface is white and smooth. The outer scales are 2 petals, crescent-shaped, thick, and close together in size. The top is flat and cracked, the base is round and blunt, and there are large scales, residual stems and heart buds each 1 piece. Hard and brittle, with white cross section and rich powder. Slight gas and bitter taste.
1 1.3.2 Fritillaria Ilicis is conical and large. The surface is slightly rough and yellowish white. The outer scales have two petals, heart-shaped, hypertrophy, one large or nearly the same size, and they are clasped. The top is slightly sharp, with few cracks and a slight depression at the base.
1 1.4 Identification (1) This product is white in powder, mainly composed of starch granules.
The starch granules of Fritillaria Sinkiangensis are oval, oval or shell-shaped, with a diameter of 5 ~ 54 μ m, and the umbilicus is punctate, herringbone or short slit, with obvious layering. There are few compound grains, which are composed of 2 fractions. Epidermal cells are rectangle-like, the vertical wall is bent like microwave, and the cells contain tiny calcium oxalate cubes. Stomatal infinitive, accessory guard cells 4 ~ 6. The diameter of the threaded catheter and the annular catheter is 9 ~ 56 μ m.
The starch granules of Fritillaria Ilicis are generally oval, triangular oval, shell-shaped or irregular round, with a diameter of about 60μm, and the umbilicus is punctate, herringbone or cross-shaped. The diameter of the catheter is about 50um.
(2) Take 5g of this product powder, add 2ml of concentrated ammonia test solution and 20ml of chloroform, shake it, leave it overnight, filter it, evaporate the filtrate, and add 0.5ml of chloroform to the residue to dissolve it as the test solution. In addition, 5g of Fritillaria Fritillaria was taken as a reference medicinal material to prepare a reference medicinal material solution by the same method, and then imperialine was taken as a reference medicinal material, and chloroform was added to prepare a solution containing 0.5mg per kloc-0/ml as a reference medicinal material solution. According to the test of thin-layer chromatography (Appendix ⅵ b), absorb 2 ~ 4 μ l of each of the above three solutions, respectively spot them on the same silica gel G thin-layer plate prepared with 2% sodium hydroxide solution, and use the lower layer solution of chloroform, ethyl acetate, methanol and water (8: 8: 3: 2)10℃ as the developing agent, unfold, take it out, dry it, and spray dilute potassium bismuth iodide in turn. In the chromatogram of the test sample, spots with the same color appear at the positions corresponding to the chromatogram of the control medicinal materials; In the position corresponding to the chromatogram of the reference substance, the same brown spots appear.
1 1.5 inspection 1 1.5. 1 moisture shall not exceed 15.0% (appendix ⅸ h first method).
1 1.5.2 the total ash content shall not exceed 4.5% (appendix ⅸ k).
1 1.6 The extract shall be determined by the cold immersion method under the water-soluble extract determination method (Appendix X A), with 70% ethanol as the solvent, not less than 9.0%.
The content of 1 1.7 was determined by high performance liquid chromatography (appendix ⅵ d).
1 1.7. 1 chromatographic conditions and system applicability test with octadecylsilane bonded silica gel as filler; Acetonitrile-water-diethylamine (55: 45: 0.03) was used as the mobile phase. Detected by evaporative light scattering detector. The number of theoretical plates should be not less than 3000 according to the peak of sibelium glycoside.
1 1.7.2 Preparation of reference solution Take appropriate amounts of imperialine glycoside reference substance and imperialine reference substance, weigh them accurately, and add methanol to make a mixed solution containing 0.2mg per 1ml.
1 1.7.3 Preparation of test solution Take the powder of this product (passing through No.4 sieve) about 1g, weigh it accurately, put it in a round-bottomed flask, add 2ml of concentrated ammonia test solution for 1 hour, and add 20ml of mixed solution of chloroform and methanol (4:1) accurately, and weigh it. Use the mixed solution of chloroform and methanol (4:1) to make up the lost weight, shake it evenly, filter it, accurately measure the continuous filtrate 10ml, evaporate it to dryness, dissolve the residue with methanol, transfer it to a 2ml volumetric flask, add methanol to the scale, and shake it evenly.
1 1.7.4 Determination Method Accurately suck the reference solution 10μl and 20μl respectively, inject 20μl of the test solution into a liquid chromatograph, determine, and calculate with the external standard two-point logarithmic equation.
The total amount of imperialine glycoside (C33H53NO8) and imperialine (C27H43N03) in this product shall not be less than 0.070% based on the dried product.
1 1.8 Sexual taste and meridian tropism are bitter, sweet and slightly cold. Return to lung and heart meridian.
1 1.9 Function and indications: clearing away heat and moistening lung, resolving phlegm and relieving cough. It is used for dry cough due to lung heat, dry cough with little phlegm, cough due to yin deficiency, and cough with blood.
Usage and dosage of 1 1. 10 are 3 ~ 9g.
11.11Note that it should not be used with Radix Aconiti, Radix Aconiti Preparata, Radix Aconiti Kusnezoffii, Radix Aconiti Preparata and Radix Aconiti Lateralis.
1 1. 12 Store in a ventilated and dry place to prevent moth.
1 1. 13 origin