1. Anticoagulant effect: 300% fungus decoction 1ml/100g was administered orally for 20 days. The experimental results showed that fungus can prolong the kaolin partial thromboplastin time by 12.06s, increase plasma antithrombin III activity, and has obvious anticoagulant effect. effect. Black fungus polysaccharide 50mg/kg has obvious anticoagulant effect when injected intravenously, intraperitoneally or orally into mice. In in vitro experiments, black fungus polysaccharide also has strong anticoagulant activity.
2. Anti-platelet aggregation effect: The phosphate buffered saline extract of black fungus significantly inhibits platelet aggregation caused by ADP in the test tube, and blocks the release of 5-hydroxytryptamine from ADP-activated platelets below 16 μmol/L. The active ingredient of black fungus anti-platelet effect is water-soluble sexual. After a person takes 70g of black fungus orally, the platelet function begins to decrease within 3 hours and lasts for 24 hours. Agaric mycelium acid extract can significantly inhibit ADP induction in vivo (10g/kg intravenously or 10g/kg orally for 15 days in rats) and in vitro (25mg/ml, 50mg/ml and 100mg (mycelium)/ml). Rat platelet aggregation. Alcohol extract 5g/kg, 7g/kg orally administered for 15 days can significantly shorten the red blood cell electrophoresis time. The results of the intraperitoneal injection test of black fungus acidic heteropolysaccharide in mice showed that the polysaccharide has the effects of promoting the increase of white blood cells, anticoagulation and reducing platelets. Its activity increases with the decrease of polysaccharide molecular weight and uronic acid content, that is, the biological activity depends on Solubility of polysaccharides in water.
Anti-thrombotic administration of 18.5 mg/kg of fungus polysaccharide to rabbits can significantly prolong the formation time of specific thrombus and fibrin thrombus, shorten the length of thrombus, reduce the wet and dry weight of thrombus, reduce the number of platelets, and reduce Platelet adhesion rate and blood viscosity, and can significantly shorten the dissolution time of guinea pig euglobulin, reduce plasma fibrinogen content, and increase plasmin activity. The results show that fungus polysaccharide has obvious anti-thrombotic effects.