GB/T

5009. 192-2003

Residue of clenbuterol in animal food

Lean meat powder

decision

Gas chromatography-mass spectrometry

The advantage of GC-MS method is that it organically combines the high-efficiency and rapid separation effect of chromatography with the high-sensitivity qualitative analysis of mass spectrometry, and can be used for qualitative and quantitative analysis of a specific residue in the presence of multiple residues, with a high detection limit. Fent

C.

A et al. used GC-MS to detect the residue of CLB in cow hair, and the minimum detection limit was 5 ng/g [6]; Peter

Batioens used gas chromatography-tandem mass spectrometry (GC-MS-MS) to detect the CLB content in cattle, sheep and pigs, and the minimum detection limit was 2ng/g[7]. Liu Qi et al. used GC-MS (EI ion source) to detect CLB in pig urine, and the detection limit was 0.5ng/mL. VanRhijin et al. used trimethylsilyl or 2- dimethylsilyl morpholine derivatives to detect CLB in urine extract, and the derivatives were scanned by electric pulse or chemical ionization, which would produce high sensitivity. In addition, compared with HPLC, GC-MS has higher detection sensitivity and lower false positive rate. Therefore, China adopts GC-MS method to detect chlorine.

Confirmation method of B (NY/T468~200 1).

High performance liquid chromatography (HPLC)

HPLC is suitable for the determination of thermally unstable and strongly polar β -agonists and their metabolites. Moreover, HPLC can be combined with pre-column extraction, purification, post-column fluorescence derivatization and mass spectrometry, which is easy to realize the automation of analysis process. Huang Shixin et al. (1995) used an ultraviolet detector to detect CLB residues in pig liver and pork. The detection condition was λ=243nm, the chromatographic column was Shimpakklc-ODS150× 6.0 Mn, the flow rate was 1mL/min, and the column temperature was room temperature: 30. Someone abroad has applied HPLC.

(Diode array detector) is used to determine CLB residues in animal food, with the minimum detection limit of 65438 0.26 ng/g and the recovery rate of 98.9%[5]. At present, high performance liquid chromatography (HPLC) has been used as a semi-confirmatory method to detect CLB residues in China. The minimum detection limit is 1 ~ 15 ng/g, which has the advantages of good specificity, strong selectivity, high detection accuracy and low false positive rate. The disadvantage is that the sample processing time is long, the detection process is cumbersome and difficult to operate, and expensive instruments are needed, which is limited in practical application.

Enzyme-linked immunosorbent assay (ELISA

)

Using the specific binding of immune antigen and antibody and the efficient catalysis of enzyme, plant horseradish peroxidase (HRP) was chemically combined with clenbuterol (CL) to form enzyme-coupled clenbuterol. The antibody coated on the solid carrier (goat anti-rabbit IgG antibody) is combined with the specific anti-clenbuterol antibody, and then the clenbuterol to be tested and enzyme-linked clenbuterol are added to compete with the clenbuterol antibody. After washing, the substrate is added, and the amount of clenbuterol to be tested is determined according to the change of colored substances. If clenbuterol is to be detected, the amount of clenbuterol coupled by binding enzyme is less, and the amount of colored substances is also less. The content of clenbuterol in the sample was determined by visual inspection or colorimetry, and the optimum wavelength of colorimetry was 450.

Nm, and the reference wavelength should be greater than 600.

Nano.

Liquid chromatography-mass spectrometry/mass spectrometry

See serial number.

1924-2007

Determination of clenbuterol, ractopamine, salbutamol and terbutaline residues in food of animal origin for import and export.

This standard is applicable to the determination of clenbuterol, ractopamine, salbutamol and terbutaline residues in muscle and viscera of animal-derived food.

The drug residue in the sample was extracted with ammonium acetate buffer with pH5.2, and then β -glucuronidase hydrochloride-arylthioesterase was added for enzymolysis. The extract was purified by C 18 and SCX double solid phase extraction columns, determined by liquid chromatography-mass spectrometry and quantified by internal standard method.

I hope it will help LZ! !

O (∩ _ ∩) O is expected.