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Which groups should be set up for methodological verification of TLC identification of traditional Chinese medicine preparations? Try to explain its purpose.
Methodological verification of TLC identification of traditional Chinese medicine preparations requires the establishment of the following groups:

1. sample group: the sample to be tested, which is used to check whether it meets the specified quality standards.

2. Control group: including positive control group and negative control group. Positive control is a standard with known composition and content, which is used to verify the reliability and accuracy of TLC. The negative control is a sample that does not contain the components to be tested and is used to exclude interference factors.

3. Repeatability group: the same batch of samples were tested at different times, different operators and different instruments to verify the repeatability and stability of the method.

4. Intermediate value group: a certain proportion of intermediate value is added between the positive control and the sample to be tested to verify the linear range of the method.

5. Interference substance group: Add possible interference substances, such as pigments and preservatives, into the sample to be tested to verify the selectivity and specificity of this method for interference substances.

The purpose of setting up these groups is to verify whether TLC as an analytical method has reliability, accuracy, repeatability, stability, linear range and selectivity specificity. Systematic verification and comparison of these groups can ensure the reliability and accuracy of TLC identification of traditional Chinese medicine preparations.

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