Weigh the dry medium powder according to the instructions of the medium manufacturer, add 1?L of distilled or deionized water, stir, heat and boil until completely dissolved

Pack into Erlenmeyer flasks and autoclave at 121°C Incubate the bacteria for 15 minutes, freeze to 45~50℃,

Add 5.0~10.0ml of sterile defibrinated sheep blood per 100.0ml of culture medium, mix well and pour onto the plate. Vaccination is required according to relevant standards.

Note:

Pre-prepared plates should not be left at room temperature for more than 4 hours before drying, and should not be refrigerated at 4°C for more than 7 days;

When the plate color changes When it turns dark red, the plate has deteriorated and should not be used;

It is best to observe the hemolytic circle through light