(I) appetizing soup. Poria 15 grams, 12 grams of Chinese yam, 30 grams of grain malt, fresh and dry gizzard each 1, boiled soup and drink. Treatment of pediatric indigestion, do not think of food.
(2) Poria barley porridge. Poria, Job's tears 25 grams each, Chen Pi 5 grams, round-grained rice, porridge. The treatment of children's spleen deficiency diarrhea, urination is not conducive.
(3) Poria barley cake. Poria, Job's tears, 30 grams of white flour, sugar, research into fine powder and evenly pressed into the cake, steamed. Suitable for children to eat, and the effect of the spleen and stomach.
(D) Poria Chenpi ginger tea. Poria 25 grams, Chen Pi 5 grams, water decoction, add ginger juice 10 drops when drinking. Have the effect of strengthening the spleen and stomach, can cure pregnancy vomiting.
Poria's role:
1. diuretic effect: 1.1. Poria raw medicine with 70% alcohol cold immersion, the use of alcohol will be immersed in the liquid alcohol evaporation, diluted with distilled water to a certain concentration, and then select healthy rabbits by body weight injection of the drug, the results of the chronic experiments show that there is a significant increase in the amount of urine after the use of the drug.
1.2. Poria decoction (0.048g/kg) was injected intravenously into dogs, the result was that the urine output did not increase, and it was also ineffective or very weak in rats; with the urinary output and chlorine excretion as the observation index, Poria decoction was given to rats (fasted for 12 hours) by gastric gavage, and the result was that Poria could not show its diuretic chlorine-excretion effect under the condition of this experiment.
1.3. Poria does not have anti-deoxycorticosterone effect.2. Antibacterial effect: 100% decoction of Poria cocos inhibited Staphylococcus aureus, Escherichia coli, and Escherichia contortus by the plate-hole method. Poria cocos had no inhibitory effect on the bacteriostatic test using test tube method. The ethanol extract of Poria was able to kill Leptospira in vitro, but the aqueous decoction was ineffective.
3. Effects on the digestive system: Poria has a direct relaxing effect on the isolated intestinal tubes of rabbits, and has a preventive effect on the ulcers formed by pyloric ligation in rats, and can lower gastric acid. In addition, it has obvious protective effect on liver injury caused by CCl4 in rats, which significantly reduces the activity of ghrelin transaminase and prevents necrosis of hepatocytes.
4. Anti-tumor effects: 4.1 The main component in Poria cocos is Poria polysaccharide (Pachyman), which has a high content. Poria polysaccharide itself has no antitumor activity, if you cut off the β-(1→6) glucopyranose branched chain contained therein, it becomes pure β-(1
4.2 Different routes of administration: Poria polysaccharide system with a variety of different routes of administration, the effect of tumor inhibition is also not the same. In the tumor inhibition test of Carboxymethyl Poria polysaccharide on Sarcoma 180, Swiss mice were given 5mg/kg dose for 10 days, the tumor inhibition rate was 99.1% by intraperitoneal injection; 96.5% by intramuscular injection; 99.6% by intravenous injection; 86.3% by subcutaneous injection; and 8.8% by oral intake.
4.3 Different strains of mice: Fungal polysaccharides were tested in different strains of mice or mongrel mice, and their tumor suppression effects varied greatly. For example, if Lentinan is tested with ICR/TCL, ddys, SWM/MS, and C57BL/6 strains of mice, a strong tumor inhibitory effect occurs, and its tumor inhibition is 85-99%; if the C3H/He strain of mice is used to do the experiments, its tumor inhibition rate is 37-48%, and if the BALB/C and DBA/2 strains are used to do the experiments, the method is according to In 1978, the national unified antitumor drug in vivo screening procedures (draft), the results of sarcoma 180 had appeared to be greater than 30% of the tumor inhibition rate, but also appeared 29.6%, 18.9% of the tumor inhibition rate and the phenomenon of no tumor inhibition; the inhibition of the mouse tumor U a 14 is not obvious. And with the same new carboxymethyl poria polysaccharide injection, with 500mg/kg, 100mg/kg, 50mg/kg, 25mg/kg dosage for the test, the results of ICR/JCL mice tumors U a 14 have a tumor inhibition effect, and its tumor inhibition rate of 75.5-92.7%.
5. The effect of Poria polysaccharides on immune function: 5.1 Increase the cytotoxic effect of macrophages: Poria polysaccharides, hydroxyethyl Poria polysaccharide-3, hydroxyethyl Poria polysaccharide-4, intraperitoneal injection can significantly enhance the cytotoxic effect of peritoneal exudate cells (PEC) in mice; Poria polyglucosides, hydroxyethyl Poria polysaccharide-1, hydroxyethyl Poria polysaccharide-2 also have a certain effect. Tests with the novel carboxymethyl Poria polysaccharide showed that it could enhance the cytotoxic effect of PEC and increase the phagocytosis rate and phagocytic index of phagocytes significantly. Subcutaneous continuous injection for 5 days, 50mg/kg dose increased the phagocytosis rate of peritoneal macrophages by 35.5% and phagocytic index by 58.O%; subcutaneous continuous injection for 10 days, 50mg/kg dose increased the phagocytosis rate by 66.1% and phagocytic index by 121%. The novel methylporin polysaccharide also antagonized the inhibition of macrophage function by the immunosuppressant cortisone acetate. The mice were given continuous subcutaneous injection of novel carboxymethyl poria polysaccharide for 10 days (50 mg/kg.day), and the cortisone control group and the carboxymethyl poria polysaccharide plus cortisone test group were both subcutaneously injected with cortisone acetate (100 mg/kg.day) for 3 days from the 8th day onwards, the phagocytosis rate and phagocytic index of the cortisone control group were 18.86±3.40% and 0.41±0.09; that of the test group was 18.86±3.40% and 0.41±0.09; that of the test group was 34.81±1%. phagocytic index were 34.81±1.75% and 0.86±0.07.The novel hydroxymethylporin polysaccharide normalized macrophage phagocytic hypophagy in Lewis lung cancer C57 pure line mice and Holoblast 180 Swiss mice.C57 pure line mice were subcutaneously inoculated with Lewis lung cancer cells for 24 minutes, and then injected with continuous subcutaneous injection of the novel carboxymethylporin polysaccharide (50mg/kg/day) For 10 days, the results of phagocytosis rate and phagocytic index were (1) normal mouse group: 39.60±4.86% and 0.99±0.20; (2) group with Lewis lung cancer: 24.44±3.38% and 0.55±0.10; group with Lewis lung cancer plus novel carboxymethyl poria polysaccharide: 59.26±6.50% and 1.30±0.21.Swiss mice After subcutaneous inoculation of sarcoma 180 cells for 24 hours, subcutaneous injection of novel Poria cocos polysaccharide (50 mg/kg /day) for 10 days, the results of phagocytosis and phagocytosis index, respectively: (1) Hormone group: 26.88 ± 4.57% and 0.74 ± 0.11; (2) Hormone plus novel Poria cocos polysaccharide group: 45.92 ± 4.13% and 1.57 ± 0.12. Other pharmacological experiments showed that the novel carboxymethyl poria polysaccharide could significantly increase the phagocytosis rate and phagocytic index of mouse peritoneal macrophages. When mice were injected intraperitoneally with the novel carboxymethyl poria polysaccharide (300 mg/kg/day) for 7 days, the phagocytosis rate of peritoneal macrophages was 38±2.46%, and the phagocytic index was 0.67±0.04, while the phagocytosis rate of the control group was 19.4±1.27%, and the phagocytic index was 0.32±0.02.
5.2 Hydroxymethyl poria polysaccharide significantly enhances the secretion of mouse splenic antibody cell count (PFC) as well as specific antigen binding cell count (SRFC). The enhancement effect of Poria cocos polysaccharide on PFC and SPFC increased with the increase of dose; it could significantly enhance the delayed hypersensitivity induced by BSA in mice. Poria hydroxymethyl polysaccharide 100mg/kg/day by intraperitoneal injection for 4 days, mice BSA-induced DTH response significantly enhanced compared with the control group, P<0.01 difference was significant; Poria hydroxymethyl polysaccharide can significantly enhance the growth of splenic T cell growth factor (TCGF) in mice. Poria hydroxymethyl polysaccharide 100mg/kg.day via intraperitoneal for 4 consecutive days, mice splenocytes stimulated by ConA to generate the amount of TCGF is significantly higher than the control group, the amount of TCGF generated by the drug group of mice compared with the normal mice increased by 3.5 times.
5.3 Increase the number of acidic non-specific esterase (ANAE)-positive lymphocytes. Poria polysaccharide was prepared into three concentrations of 250mg/kg/day, 500mg/kg/day and 1000mg/kg/day by gavage, and was administered for 7 consecutive days. On the 8th day, the number of ANAD-positive lymphocytes, hemolymphatic vacuoles (PFC) and macrophage phagocytosis were detected, and the weights of the thymus, spleen and tumors were also measured, which proved that poria polysaccharide could enhance M phagocytosis. polysaccharide could enhance M phagocytosis (P<0.01) and increase the number of ANAE positive lymphocytes (P<0.05). It also significantly increased the number of antibody-secreting cells in mouse spleen (P<0.01), and had the functions of anti-thymic atrophy and anti-spleen enlargement and tumor growth inhibition.
5.4 Enhancement of cytotoxicity of T-lymphocytes: Poria polysaccharides can enhance the cytotoxicity of T-lymphocytes, i.e., enhance the cellular immune response, and consequently activate the body's immune supervisory system against tumors, which is closely related to its anti-tumor activity.
5.4.1.In-tube test: target cells were labeled with 51Cr, lymphocytes cultured for 5 days were mixed with labeled target cells at 1:1, and the number of 51Cr released was used as an indicator of target cell damage.
The cytotoxicity of lymphocytes was observed accordingly. Different doses of Poria polysaccharide were added to the test to observe the effect on the cytotoxic effect on lymphocytes. The results showed that Poria polysaccharides and hydroxyethyl poria polysaccharides enhanced the cytotoxicity of lymphocytes by 20-28 times in the tube, and Poria polysaccharides and carboxymethyl poria polysaccharides enhanced it by 4-7 times.
5.4.2. In vivo test: CBA mice were used for the test, and P815 tumor cells were used as lymphocyte activators at the beginning to sensitize them, and various Poria polysaccharides were injected intraperitoneally on different days. The mice were put to death on day 10 and splenocytes and mesenteric lymphocytes (MLNC) were removed. The 51Cr-labeled P815 tumor cells were used as target cells for cytotoxicity of T-lymphocytes, which were mixed with the prepared splenocytes and MLNC at a certain ratio (l : 100) and cultured for 3 hours to observe the release rate of 51Cr. The results proved that all kinds of Poria cocos polysaccharides could enhance the cytotoxic effect of T lymphocytes in vivo. In addition, 100 μg/ml of hydroxymethyl Poria polysaccharide had a promoting effect on NK cell activity.
6. Effects on blood system: 6.1. It can accelerate the recovery of leukopenia in rats caused by cyclophosphamide.
6.2. The aqueous extract of Poria containing water-soluble small molecular polysaccharides can increase the level of 2,3 a DPG of isolated healthy human erythrocytes by about 25%, and can effectively delay the depletion of 2,3 a DPG in the process of warming; the overall level of 2,3 a DPG of the mice administered intravenously increased significantly.
6.3. Poria cocos decoction administered by subcutaneous injection and administered by gavage significantly increased plasma corticosterone in mice.
7. Effects on central nervous system: Poria cocos decoction administered by intraperitoneal injection at the rate of 5-10g/kg had a sedative effect on mice given caffeine beforehand or not given caffeine. Synergistic effect of Poria cocos with sodium pentobarbital. Poria decoction (10g/kg) failed to significantly prolong anesthesia with sodium pentobarbital; with a dose of 40g/kg it resulted in a more significant prolongation of anesthesia compared to the control, and the sedation index increased as the dose was increased. This synergistic effect may be due to their central inhibitory effect, or it may be due to the prolongation of anesthesia by impeding the breakdown and excretion of pentobarbital.