Cultivation techniques:
(1) Liquid medium formula suitable for medium formula:
Formula 1: protein knee 2%, sucrose 2%, magnesium sulfate 1.5%, potassium dihydrogen phosphate 0.3%, vitamin b11mg/100 ml, and the pH is adjusted to 6.
Formula 2: yeast extract 0. 1%, sucrose 3%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 1%, sodium nitrate 0.3%, potassium chloride 0.05%, and pH adjusted to 6.
The above two formulas are from the Department of Biology, West China University of Medical Sciences to Cheng Yu et al., 19880.
③: 0. 1% yeast extract, 3% sucrose, 0. 1% potassium dihydrogen phosphate, 0.5% magnesium sulfate, 0.3% sodium nitrate and 0.05% potassium chloride.
(2) Submerged culture process: slant mother seed ~ primary shake flask seed (250ml triangular flask, 50ml liquid, 25℃-28℃, 120 rpm, 2-3 days) ~ secondary shake flask seed (500ml triangular flask, inoculation amount 5%). , . 10%).
(3) separating, concentrating and drying the liquid fermentation cultured mycelium, putting into a tank, separating the mycelium with a plate-and-frame filter press or a centrifuge, putting into a vacuum concentration tank for decompression, concentrating at 60-80 DEG C, and drying at 80 DEG C to obtain the mycelium.
(4) The chemical composition of the dried mycelium was analyzed, and the approximate content was: protein 9.2%, fat 8. 5%, carbohydrate 0.8%, ash 3.9%, calcium 36.8 mg/100g, phosphorus 5.0 mg/100g.
Growth and development conditions:
(1) Nutrition is the main source of nutrition for the growth and development of wild termites, which comes from ant nests, fungus gardens and the surrounding environment.
(2) Temperature: The temperature in the termite nest where Pleurotus ostreatus grows is stable at 22℃ 3℃, the lowest is 65438 05℃ and the highest is 28℃, which is almost constant temperature.
(3) The humidity and stable environmental conditions in the ant nest are suitable for the growth of Pleurotus ostreatus mycelium, but not conducive to the formation of fruiting bodies.
(4) The pH value of the fungus garden in the ant nest is relatively stable, often at 4.0-4.5. Chemical analysis showed that Wang Anyuan, who caused the pH value of ant A to be 1, was born with ten kinds of organic acids, including volatile formic acid, nonvolatile aconitic acid, fumaric acid and succinic acid.
(5) The concentration of carbon dioxide in air ant nests is generally 3%-5%, which is more than 100 times higher than the normal air content. Pay attention to ventilation when breeding artificially.
(6) Light is not necessary for spore germination, mycelium growth, primordium differentiation and fruiting body growth and development. When the fruiting body opens its umbrella, it needs some scattered light to make it differentiate and develop well.