Agarose gel electrophoresis can identify the detected dna by restriction endonuclease polymorphism (rflp) or amplified fragment polymorphism (aflp). That is, different dna fragments cut by specific restriction enzymes have different sizes, and the bands run out by agarose gel electrophoresis are also different. If it is the same dna, the result is the same.

principle

The main difference between agarose gel electrophoresis and other support electrophoresis is that it has dual functions of "molecular sieve" and "electrophoresis".

Agarose gel has a reticular structure, and the molecules of the substance will be resisted when passing through, and the macromolecules will be greatly resisted when surging. Therefore, in gel electrophoresis, the separation of charged particles depends not only on the nature and quantity of net charge, but also on the size of molecules, which greatly improves the resolution. However, because its pore size is too large compared with that of protein, its molecular sieve effect is negligible for most protein, and it is widely used in nucleic acid research.