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Identification method of Aristolochia
Shape discrimination

The capsule is spherical or rectangular, with a blunt base and a straight longitudinal edge of the back seam. Seeds slightly wider than long, heart-shaped. The huge Aristolochia is huge and yellow-green, and it is not easy to break.

Microscopic identification

1. There is one layer in the cross section of the fruit flap, and the cells in the other layer are rectangular or quasi-circular. 2. The outermost layer of the cross section of the seed coat is 1 row of brown cells, and inward is 1 row of lignified cells with square crystals.

Physical and chemical identification

1. Drop the ethanol extract of this product on the filter paper. Observed under ultraviolet lamp, it shows yellow-green fluorescence. 2. Take 3g powder of this product by TLC, add 50ml ethanol, heat it in water bath for 65438±0h, and filter. The filtrate was evaporated to dryness, and the residue was dissolved in 5ml ethanol as the test solution, and the ethanol solution of Aristolochia was used as the control solution. Take 3μl of the above two solutions and spot them on the same silica gel G thin-layer plate. Using chloroform-acetone and glacial acetic acid (8:5:0. 1) as developing agent, take it out, dry it in the air, and test it under fluorescent lamp. The test sample and the reference sample show spots of the same color at corresponding positions.

chemical composition

Contains aristolochic acid, magnolin, tetrandrine, etc.