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The bottom of each bottle of culture medium is solidified very hard, but the top is not solidified. Why?
There are many reasons why agar doesn't coagulate, such as low pH value, too little agar, uneven stirring of culture medium, poor quality of agar, and too long disinfection time, which may all cause non-coagulation. However, the phenomenon of uneven coagulation you mentioned may be due to insufficient stirring of culture medium during heating. So that the agar is unevenly distributed.