Single molecule counting (SMC) is a method to quantitatively detect and count specific kinds of single molecules in solution samples, so as to detect the solution concentration.

SMC？ Single molecule immunoassay technology has the characteristics of wide dynamic detection range of 4 logs, flexible selection of experimental scheme according to target abundance and cost, convenient development of detection kit and easy use close to ELISA.

Extended data

Main characteristics of SMCTM single molecule immunoassay technology;

1, breakthrough ultra-high detection sensitivity

SMC？ Single-molecule immunoassay is as simple as enzyme-linked immunoassay, but its sensitivity can reach about 1000 times that of ELISA, and the protein target with fmol concentration has been successfully detected. High-energy laser is focused on a single fluorescein-labeled secondary antibody molecule in solution through Airy spot, and the fluorescence flicker generated is digitally measured, thus realizing "single molecule detection".

The new single molecule detection method is much more sensitive than the traditional ELISA, Multiplex and other technologies, which makes the detection of unknown protein targets far more than known proteins become a reality, and will bring new foundations and new discoveries in translational medicine.

2. Large dynamic detection range up to 4 logarithms.

Taking Erenna as an example, the dynamic detection range is greater than 4 logs, which is beneficial to the simultaneous detection of samples with great differences in protein target expression.

3. Choose the experimental scheme flexibly according to the target abundance.

Both magnetic beads and ordinary 96-well plates can be used as antibody coating media. It strikes a clever balance between sensitivity and cost.

Baidu encyclopedia -smc(SMC single molecule immunoassay technology)