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How to determine amino acid nitrogen in vinegar by national standard method?
The "quick detection box for food safety" and "quick detection box for common acute food poisoning" were developed by the Institute of Nutrition and Food Safety of China Center for Disease Control and Prevention and produced by Beijing Zhi Lin Science and Technology Company. They are small, dignified and easy to carry. The storage box in the box is not only large in capacity, but also can be combined at will. The detection items in the detection box are all aimed at the key and difficult points of social concern and the key links that are prone to problems in the food chain. Among these methods, except microbial method, the average detection time of the project is less than 10 minute, and the results can be produced within 30 minutes at the longest, which is very suitable for field use.

The food safety quick detection box consists of an outer box, a male box, a detection box and auxiliary instruments. The outer color of the detection box is black and silver gray, and the inside of the upper cover is equipped with a standard test bag, which contains common equipment, such as multifunctional scissors, pipettes, measuring cylinders, etc. The inspection box is made of materials with acid and alkali resistance, impact resistance and extrusion resistance, which is reasonable in design and convenient for carrying and field work. The food safety quick detection box has decent appearance design, appropriate size and convenient carrying. The design of the storage box in the box is not only large in capacity, but also can be combined at will. The detection project is aimed at the hot and difficult points of food safety that the society pays attention to and the key links that are prone to problems in the food chain. At present, the test equipment is relatively advanced or suitable in China.

Sources of detection methods: First, many reagents used in national standard method, AOAC method or classic textbook method are made into kits, kits or test paper cards in advance to minimize the configuration of on-site reagents. The instruments and appliances used in this method have been developed or carefully selected into some small and portable devices. The second is to transform the latest scientific research achievements in the laboratory into testing equipment and methods suitable for field use. Some of the rapid detection methods used are developing our national standards, such as the rapid detection of nitrite in vegetables, and some have become national standards, such as the detection of coliform bacteria and the rapid detection of pesticide residues.

At present, there are more than 40 physical and chemical rapid detection items in the food safety rapid detection box, which are increasing with the development of social demand and technology. The operation method is simple and easy, which brings convenience to operators and provides scientific and effective means for food safety supervision, quality control and rapid screening of food poisoning.

Brief introduction to the configuration of food safety rapid detection box

The food safety rapid detection box is divided into: simplified configuration, mid-range configuration, high-grade configuration and microbial part. It can be equipped in complete sets or configured as required.

1. Thin configuration (two boxes, 38 test items)

Basic configuration of 1. 1

product name

Technical specification of products

product name

Technical specification of products

Artificial leather or aluminum alloy box

1

300ml plastic liquid storage bottle

1

Micro electronic balance

1 unit

filter paper

1 box

calculator

1

chimney

two

Storage box

five

Pesticide extraction tank

10

test-tube rack

1

Disposable dropper

10 branch

Multifunctional scissors

1

Medicine spoon

three

colorimetric tube

Five branches

PH test paper

1 Bao

Size test tube

Five branches

Miniature water bath pot

1

Each 5ml pipette ball

1

explain

1 serving

100ml plastic liquid storage bottle

1

1.2 inspection project configuration

product name

Technical specification of products

product name

Technical specification of products

Food center thermometer

a sheet

electric conductivity meter

1 branch

Rapid alcohol measuring box

1 set

Rapid detection card for pesticide residues

2 boxes (40 copies)

Rapid measuring tube for nitrite

40 doses

Rapid measuring box for sulfur dioxide

About 100 parts.

Formaldehyde qualitative rapid detection kit

100 parts dosage

Formaldehyde qualitative rapid measuring tube

15 parts dosage

Sudan red detection kit

50 doses

Water injection meat test paper

60 doses

The dosage of 50 parts of milk powder protein rapid detection box

Clenbuterol rapid detection card

10 dosage

Arsenic and mercury detection reagent

About 50 doses

Cyanide detection reagent

About 20 doses.

Cyanide detection device

1 set

Rapid detection and identification reagent for hemp oil in edible oil

About 10 parts.

Rapid determination card for acid value and peroxide value of edible oil

10 dosage

Rapid detection and identification reagent for croton oil in edible oil

About 10 parts.

Rapid detection and identification reagent for tung oil in edible oil

About 50 doses

Rapid detection and identification reagent for mineral oil in edible oil

About 10 parts.

Rapid detection solution of fake and shoddy monosodium glutamate

About 20 doses.

Rapid detection test paper for free inorganic acid in vinegar

40 doses

Test solution package for rapid determination of total acid in vinegar

About 25 doses

Liquid package for rapid detection of total acid and amino acid nitrogen in soy sauce

About 25 doses

Rapid detection test paper for available chlorine and hydrogen peroxide in disinfectant.

1 box

Rapid determination solution for iodine content of iodized salt

(About 500 copies)

Rapid detection technology of food safety (book)

1 serving

Statistical software for physical and chemical inspection data

1 sheet

Lecture on food safety testing technology (CD)

1 sheet

2. Mid-range configuration (three boxes, 4 1 test item)

On the basis of thin configuration, add:

1 rapid pesticide residue detector (box)

Manually adjustable pipette 1.0 ~ 5.0ml (made in China, Finland) 1 piece.

1 precision acidity meter (with calibration reagent) and 1 handheld indoor and outdoor electronic thermometer.

Vehicle power converter 1

Rapid determination box for free residual chlorine 1 box.

3. Advanced configuration (four boxes, 45 inspection items)

On the basis of mid-range configuration, add:

Far infrared thermometer special for food and environment

Ultraviolet-assisted illuminance meter in disinfection room

Meat moisture detector

Portable ultrasonic extraction, dissolution and cleaning device

1 micro centrifuge (set)

1 food grinder

Dual channel timer 1

1 set of arsenic detection tubes for health indicators

Change imitation suitcase into suitcase.

Add 1 aluminum alloy box.

Second, part of the test project introduction

(a) rapid screening and determination of acute food poisoning substances

1. pesticide determination: rapid test card method (national standard method), qualitative and semi-quantitative. It is mainly used for rapid qualitative determination of organophosphorus and carbamate pesticide residues in vegetables. The positive results exceeded the standard, and the coincidence rate was about 80%. After selecting the speed measuring instrument designed for the speed measuring card, 10 samples can be detected within 15 minutes. This method is also suitable for rapid screening and identification of food poisoning substances.

2. Determination of rodenticide: reagent package method (laboratory scientific research results), qualitative detection. Within 30 minutes, four rodenticides (tetramine (0. 1μg/ml), fluoroacetamide (50μg/ml), diphacinone sodium salt (45μg/ml) and spironolactone (20μg/ml) can be identified. Mainly used for preventive monitoring, screening and qualitative identification of toxic substances. (Detection limit in brackets).

3. Determination of nitrite: rapid tube method (rapid method based on national standards), qualitative and semi-quantitative detection. It can be used for health index detection, poisoning monitoring, rapid screening and qualitative and quantitative analysis of food poisoning substances. The minimum detection limit is 0.025 mg/L. Field use, 15 minutes to get the result.

4. Determination of methanol: fast alcohol detector (patented method), quantitative determination without reagent, giving the result within 10 minute. It is mainly used for the determination of methanol (≥ 1% V/V) in mixed wine, especially for the rapid determination of wine samples that can cause acute methanol poisoning. The invention is suitable for the on-site rapid determination of the content specified by national standards in distilled liquor, and is also suitable for the rapid determination of the methanol content in secondary distilled mixed liquor.

5. Determination of arsenic and mercury: Toolbox method (classic traditional method) is mainly used to prevent highly toxic arsenic compounds represented by arsenic trioxide (arsenic) and highly toxic mercury compounds represented by mercury chloride from being mixed into food, and to monitor and identify toxic substances. The detection limit of arsenic is 65438±0μg/g/g, and that of mercury is 20μg/g, which is basically qualitative within 30 minutes.

6. Rapid characterization of cyanide: Picric acid test paper method (national standard method) is mainly used to prevent the mixing of highly toxic cyanide, monitor the mixing of cyanide into food, and screen and identify toxic substances. On-site testing results in 20 minutes. In the sample of 10g, the detection limit of picric acid test paper for cyanide is 0. 15mg, which is equivalent to 15mg/kg.

7. Determination of acid value and peroxide value of edible oils and fats: rapid card method (patented method), semi-quantitative detection, acid value detection range of 0 ~ 5.0 mg KOH/g, peroxide value detection range of 0 ~ 50 eq/kg, and the results are obtained within 2 minutes. It is mainly used to monitor the hygienic index of edible oil and judge whether the edible oil is fresh or rancid.

8. Determination of non-edible oil: reagent package method (national standard method and classical method), qualitative detection. The results will come out in 5~20 minutes. Mainly used for qualitative identification of edible oils contaminated by mineral oil (0. 1%), tung oil (0.5%) and croton oil (2.5%), and screening and qualitative identification of toxic substances caused by these non-edible oils. (Detection limit in brackets).

9. Rapid qualitative determination of clenbuterol hydrochloride: colloidal gold labeling method (patented method), qualitative detection. The results will be available in 20 minutes.

(2) Rapid detection of chronic harmful substances

1. formaldehyde determination: kit or rapid test tube method (laboratory scientific research results), qualitative detection. It is suitable for the determination of artificially added formaldehyde or sodium bicarbonate in water-based products and processed foods that need to be preserved. The detection limit is 65438 00 μ g/ml. The results will be announced in 3 minutes.

2. Rapid detection of free mineral acids and senses in vinegar: paper method (national standard method), qualitative, with a minimum detection amount of 5μg and results within 5 minutes.

3. Rapid determination of bleaching agent (sulfur dioxide) in food: drop bottle method, improved on-site rapid detection method of national standard analysis method. It is mainly used for rapid determination of bleaching agent abuse in food and identification of artificial addition of sodium bicarbonate. The detection limit is 0.00 16g/kg. The detection time (based on the white sugar sample) is 5 minutes.

4. Determination of free residual chlorine: kit method (national standard rapid method), semi-quantitative, detection limit of 0.05mg/, and the result will be obtained within 5 minutes. It is suitable for the determination of residual chlorine in tableware (drinking utensils) after disinfection, free residual chlorine in drinking water at the end of pipe network and residual chlorine in artificial swimming pool water.

5. Rapid detection of Sudan red: Paper chromatography in GB/T5009.35-2003 was adopted and improved. In the case of excluding edible pigments, non-edible pigments were detected, and Sudan red 1, 2, 3, 4 were detected quickly. The minimum detection amount is Sudan red pigment visible to the naked eye. At the same time, it can also detect some other non-edible pigments.

6. Rapid detection of boric acid and borax: adopt the method of national pharmacopoeia and improve it to make it suitable for field use. It is suitable for detecting the phenomenon that boric acid or borax is mixed into grain as an insecticidal preservative. On-site inspection, completed in 10 minutes.

(3) Rapid detection of fake and shoddy food.

1. Determination of iodine content in iodized salt: one drop method (laboratory method), semi-quantitative, mainly used for determination of iodine content in iodized salt and identification of true and false iodized salt. The test range is 0 ~ 40 mg/kg. 1 min.

2. Rapid determination of total acid content in vinegar: drop bottle counting method (a rapid method based on national standard). Mainly used for on-site inspection of product quality and fake and shoddy products. The results were obtained within 5 minutes, and the method error was 0.3%.

3. Rapid determination of total acid and amino acid nitrogen content in soy sauce: drop bottle counting method (a rapid method based on national standard). Mainly used for on-site inspection of product quality and fake and shoddy products. The results were obtained in 10 minutes. The errors of total acid determination and amino acid nitrogen determination are 0.45% and 0.078%, respectively.

4. Rapid detection of fake and shoddy monosodium glutamate: drop bottle counting method (a rapid method based on national standard). The results of field detection are obtained within 5 minutes, and the error of this method is 2.6%.

5. Rapid identification of water-injected meat: rapid meat moisture analyzer (patented method) and water-injected meat monitoring test paper method can produce results within 5 minutes.

6. Rapid identification of bottled purified drinking water: conductivity measurement (national standard method), and the result can be obtained within 2 minutes.

7. Detection of adulterated auricularia auricula: detection of water absorption and pH value (laboratory method). The results will be available in 30 minutes.

8. Determination of pH value of food: test paper method and acidity meter method, the measuring range is 0 ~ 14 pH, the accuracy is: test paper method 1pH, acidity meter method 0.2pH, 1 min. It is mainly used to detect whether the pH value of food is within the specified range.

9. Rapid detection of honey concentration and water content: honey hydrometer method. The method of national industry standard GH0 12-82 was adopted and improved to make it suitable for field use. The results will be announced in 20 minutes. It is suitable for rapid detection of inferior honey and adulterated honey.

10. Rapid detection of honey acidity: drop bottle counting method. It is improved on the basis of the national industry standard GH0 12-82, making it suitable for field use. The results will be announced in five minutes. It is suitable for rapid detection of inferior honey and adulterated honey.

1 1. Rapid detection of fake and inferior auricularia auricula: detection of water absorption and pH value. It is suitable for rapid detection of adulterated auricularia auricula. The results will be available in 30 minutes.

(d) Quickly determine the safety of food processing and storage.

1. food center temperature measurement: central thermometer, measuring range -50~ 150℃, resolution 0. 1℃, accuracy 1℃. The results will be announced in five minutes. It is mainly used to monitor the temperature of food centers specified in the Measures for Hygienic Management of Catering Industry.

2. Determination of food surface and ambient temperature: far infrared thermometer (American product), with the range of -30~200℃, resolution of 0.5℃ and accuracy of 65438 0℃, and the result can be obtained within 65438 0 seconds. It is mainly used to measure the temperature of food production, storage, transportation and sales required by HACCP.

3. Determination of auxiliary ultraviolet irradiation intensity in disinfection room: The portable auxiliary irradiation instrument (standard method issued by the Ministry) can complete the detection items specified in the Hygienic Standard for Disinfection of the Ministry of Health within 10 minute.

4. Determination of available chlorine: quick card method (laboratory method), semi-quantitative, measuring range 10 ~ 300mg/L. 1 min. It is mainly used to monitor the content of available chlorine in chlorine-containing disinfectants to ensure disinfection effect.

(5) Rapid detection of microorganisms

1. determination of coliform bacteria in tableware: paper method (national standard method), field sampling for 3 minutes, and incubation in incubator for 24 hours to get the results. It is mainly used to monitor the sanitary condition of tableware.

2. Determination of coliform bacteria in food: The test paper method is suitable for rapid determination of coliform bacteria in milk, cold drinks and condiments. It takes 15 ~ 24 hours to produce results, while the traditional method takes nearly one week.

3. Determination of the total number of colonies in food: The test piece method takes 18 ~ 24 hours to produce results, while the traditional method takes 48 hours.

4. Determination of the number of molds and yeasts in food: the paper method takes 48 hours to produce results, while the traditional method takes nearly one week.

5. Determination of Staphylococcus aureus in food: test paper method, and the results will be obtained in 26 hours.

Three. Introduction of auxiliary equipment

1. Micro electronic balance

Palm size, easy to carry. The stripping is clearly displayed, and the operation is simple. The measuring range is 0. 1~200g, which can meet the requirements of field sampling and reagent weighing.

2. Micro ultrasonic extraction, dissolution and cleaning device

Small size and easy to carry. Firstly, it can be used to extract pesticide residues from vegetables, which saves time and effort and has good effect. Second, it can accelerate the dissolution of insoluble solid samples or solid reagents. Thirdly, the cleaning speed of experimental utensils can be accelerated, and the cleaned utensils have high cleanliness.

3. Micro constant temperature water bath pot

It can be used to eliminate the interference of ammonia in fluoroacetamide determination and improve the stability of test tube reaction results. You can also use the electric heating plate in the constant temperature water bath to detect arsenic and mercury, which can replace the traditional alcohol lamp, alcohol and tripod and is more convenient to carry.

4. Micro centrifuge

Small size and easy to carry. It can shorten the operation time of partial sample extraction, separation (precipitation), reduce the interference of impurities, and broaden the scope of detecting food types.

5. Manually adjustable pipettes (Finland)

The measuring range of 100~ 1000 ul is simple to operate and convenient to use, which is very suitable for field use.

6. Conductivity meter

It is not only an instrument to identify the authenticity of drinking pure water, but also a device to determine whether experimental water is available. The pen conductivity meter is easy to carry, and the handheld conductivity meter is durable.

7. Sample crusher

Portable, used for evenly crushing samples.