The volatile oil of Schizonepeta tenuifolia (0.5ml/kg) was given orally, which had a cooling effect on rats. 25, 50mg/kg intragastric administration can obviously inhibit the writhing reaction induced by acetic acid in mice; 0.2 ml/kg and 0.5ml/kg intragastric administration can inhibit the swelling of rats' feet caused by carrageenan and egg white, and also show good antagonism to the swelling of mice's ears caused by xylene, the swelling of mice's feet caused by carrageenan, the increase of mice's abdominal capillary permeability caused by acetic acid and the increase of mice's skin capillary permeability caused by xylene. It also showed inhibitory effect on the chronic inflammation model of cotton granuloma in mice. The intraperitoneal injection of 4.4g/kg decoction has antipyretic effect on rabbits with body temperature increase caused by refined tetanus toxoid mixture of typhoid and paratyphoid vaccine. 14g/kg intraperitoneal injection can inhibit the increase of abdominal capillary pain permeability induced by acetic acid in mice. 20g/kg intraperitoneal injection of decoction has inhibitory effect on ear inflammation caused by croton oil in mice. 5g/kg intraperitoneal injection has analgesic effect on hot plate method in mice. The main analgesic component of Schizonepeta tenuifolia is d- menthone, and the volatile component 3- methylcyclohexanone separated from Schizonepeta tenuifolia also has analgesic effect [3,4,16].
2 sweating effect
Histomorphological observation showed that the lipid extract from Schizonepeta tenuifolia (2 mg/kg, 4 mg/kg) was injected intraperitoneally into rats, which could obviously improve the incidence of vacuoles, number density and area density of sweat acinar epithelial cells [19].
Effect of 3 on blood system
3. 1 hemostasis Ding Anwei and others compared the hemostatic effects of raw Schizonepeta tenuifolia and Schizonepeta tenuifolia charcoal (charred by slow fire). Akonob's method and capillary method were used. Rabbits were given 2g/kg and mice 5g/kg by gavage, and the bleeding and coagulation time were measured. The results showed that raw products could not significantly shorten the bleeding time, but could shorten the coagulation time by 30%, while Schizonepeta charcoal could shorten the bleeding time and coagulation time by 72.6% and 77.7% respectively. It shows that Schizonepeta tenuifolia has hemostatic effect after charring. StE, a fat-soluble extract of Schizonepeta tenuifolia charcoal, has obvious hemostatic effect. The dose-effect relationship of Schizonepeta tenuifolia and its extract was studied, and it was found that in a certain dose range, the logarithmic dose was significantly linearly related to the coagulation and bleeding time of mice [5, 6].
It is reported that the application of Schizonepeta tenuifolia charcoal extract (StE) in vivo has no obvious effect on platelet aggregation in experimental animals. In vitro medication has a low concentration (<: 0.625mg/ml) can strongly promote platelet aggregation, but it has inhibitory effect when the concentration is higher than 5.0mg/ml, and the inhibitory effect of StE becomes stronger with the increase of concentration. The results of experimental thrombosis showed that StE had no effect on thrombosis, but the high dose group (84mg/kg) seemed to have an inhibitory tendency. The experimental results suggest that StE, a carbon extract from Schizonepeta tenuifolia, has a bidirectional effect on the blood system, that is, it not only has a strong hemostatic effect, but also shows a certain tendency to promote blood circulation at a high dose. It may be related to the fact that StE can significantly increase the plasma PGE level of experimental animals [7].
Studies have shown that the hemostatic effect of StE, a carbon extract from Schizonepeta tenuifolia, is achieved by promoting coagulation and inhibiting fibrinolytic activity in vivo. StE can significantly shorten the plasma prothrombin time (PT), thrombin time (TT), partial thromboplastin time (KPTT) and plasma recalcification time (RT) in experimental animals, and it has anti-heparin effect in vivo. At the same time, it can obviously shorten euglobulin lysis time (ELT) and enhance fibrinolytic activity (FA). Plasma protamine sulfate Paracoagulation (3P) test and ethanol gel test (EGT) were negative, which ruled out the possibility that large dose of StE could cause disseminated intravascular coagulation (DIC) [5].
3.2 Effect on Blood Viscosity: When StE emulsion (0.7%, 1.4%) is administered to rats for 5 days, it can obviously increase the whole blood specific viscosity (high shear and low shear) and RBC hematocrit of rats, but the plasma specific viscosity and RBC electrophoresis time have no obvious changes. The RBC number of animals in StE group has an upward trend, but it has no obvious effect on platelet number [1]. Intraperitoneal injection of 2.0 mg/kg, 4.0 mg/kg and 8.0mg/kg of lipid extract from Schizonepeta tenuifolia can significantly reduce the specific viscosity of whole blood, and the aggregation of red blood cells can be reduced in high dose group [19].
4 the role of pathogenic microorganisms
Li Xu et al. found that the dosage groups of 5.0mg/kg and 10.0mg/kg of Schizonepeta tenuifolia alcohol extract had significant protective effects on the mortality of mice infected with influenza A virus A/PR/8/34(H 1N 1), and the death inhibition rates reached 40% and 50%. 1.7mg/kg, 5.0mg/kg and 10.0mg/kg can obviously reduce the lung index of mice infected with H 1N 1 virus, and the inhibition rates of lung index are 26%, 30% and 3 1% respectively.