1, and the culture temperature of the strain in PDA medium is 25℃ ~ 30℃.

2. The components of the expanding culture medium are sawdust, wheat bran, white sugar, calcium phosphate fertilizer from poultry manure, monosodium glutamate and VB powder.

3. The ingredients of the cultivation material are: sawdust, humus soil, poultry manure, wheat bran, sandy soil and phosphate rock powder.

4. The method of sowing strains during cultivation is a combination of on-demand sowing and hole sowing.

5. After the primordia grow to the surface, the temperature of mushroom house should be controlled at12℃ ~17℃.

6. After the original base grows to the surface, the humidity of the mushroom house is kept above 85%.

Preservation method of Ganba bacteria:

1, fresh Ganba Yunnan can be preserved for a long time as long as it is relatively dry. Remove the soil and weeds, keep the dry weight, put it in a fresh-keeping bag, put it in the frozen layer of the refrigerator, take it out and thaw it naturally before eating, and rinse it with clear water.

2. Remove the soil-covered skin from the roots of fresh Ganba fungus, arrange it into strips with basically the same size, wash it with clear water, then soak it in salt water for 5-9 days, take it out, rinse it with clear water, put it in an impermeable container, and soak it with fresh-keeping liquid. The fresh-keeping liquid is weighed as10 ~ 25% of the total weight of Ganba fungus with water, and sodium benzoate is weighed as1~ 4 ‰. The liquid level should be slightly higher than the pile surface of Ganba bacteria, and then the container mouth should be closed to isolate air and maintain hygiene. After soaking for one month, it can be taken out and packaged as a product, and the preservation period is more than 10 month.