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Identification of total glucosides of Paeonia lactiflora
Take 0.65438 0 g of this product, add 65438 00 ml ethanol, shake well for 5 minutes, filter, evaporate the filtrate, and dissolve the residue with 65438±0ml ethanol as the test solution. In addition, paeoniflorin reference substance was added together with ethanol to prepare a reference substance solution containing 1mg per 1ml. According to the thin-layer chromatography (China Pharmacopoeia 1995 Appendix ⅵ b), respectively absorb the above two solutions 10μl on the same silica gel G plate, and use chloroform-ethyl acetate-methanol-formic acid (40:5: 10:0.2) as the developing agent, unfold, take out and dry. In the chromatogram of the test sample, the same blue-purple spots appear in the position corresponding to the chromatogram of the control sample.