The relative molecular weight of bovine serum albumin is the fourth power of 10, which is not certain, but polymer. In some places, the test data is 70 000, which is the data used in PCR. Bovine serum albumin is the main component in blood (38g/ 100ml), with a molecular weight of 68kD, an isoelectric point of 4.8, a nitrogen content of 16%, a sugar content of 0.08%, only hexose and hexosamine, and a fat content of only 0.2%. Albumin is composed of 58 1 amino acid residues, of which 35 cysteines are composed of 17 disulfide bonds, and there is a free sulfhydryl group at the 34th position of the peptide chain. Albumin can be combined with many cations, anions and other small molecules. The bovine serum albumin solution can be used as a standard curve for measuring the content of protein. Generally, the bovine serum albumin bought back is a fine flaky milky white powder. Bovine serum protein is also based on iron. Uses 1, ultra-pure bovine serum protein uses: as a sealing agent for diagnostic reagents such as enzyme labeling and gold labeling, and biochemical tests. Properties: light yellow dry powder 2. Fatty acid-free bovine serum albumin Application: It is used as a sealing agent and protective agent for diagnostic reagents such as enzyme label and gold label, especially suitable for products and tests that are easily affected by fatty acids. Properties: pale yellow dry powder 3. Protease-free bovine serum albumin Uses: It is used as a sealing agent and protective agent for diagnostic reagents such as enzyme label and gold label, especially suitable for blood lipid diagnostic tests, lipid diagnostic reagents and products with high purity requirements. Properties: light yellow dry powder 4. Cell culture grade bovine serum protein Usage: Protein additive in serum-free medium for cell culture. Properties: The standard curve for determination of light yellow dry powder albumin generally uses spectrophotometry to measure the content of substances. First, the standard curve should be made, and then the content of the measured substances should be found out according to the standard curve. Therefore, making standard curve is a basic technology of biological detection and analysis. Determination method 1. Kjeldahl nitrogen determination method 2. biuret method 3. Folin- phenol reagent method 4. Ultraviolet absorption method 5. Coomassie brilliant blue method standard curve preparation reagent 1. Coomassie brilliant blue reagent: Coomassie brilliant blue G-250100 mg is dissolved in 50ml 95% 95% ethanol, and added with/.