No.

The first villain we encountered was Doctor Octopus, and the Green Goblin also appeared, although not as many scenes. Doctor Octopus first discovered that this was not Toby. Later, villains appeared one after another. There were only the five major villains: Doctor Octopus, Green Goblin, Sandman, Dr. Lizard and Electro, and no one else. Then Brother Holland helped Doctor Octopus repair the inhibitor chip, allowing Doctor Octopus to regain control (that is to say, he became better). Doctor Strange said that he would send the villains back, but Brother Holland believed that once he sent them back, they would return to where they would die. At that moment, so he wanted to help them, several villains also unanimously begged Dutch brother, and Dutch brother was willing to help them, but later the other four betrayed him, and then the Green Goblin killed Aunt May.

The film "Spider-Man 3" mainly tells the story of Spider-Man becoming a respected American hero, but Peter, who was parasitized by the "Venom" organism, began to become arrogant. Later, Peter got rid of Venom, and join forces with friends to fight against Venom and the Sandman.