Fisheye can enhance the phagocytosis of WBC and improve serum preparin, in the treatment of chronic bronchitis, the synthesis of fisheye can make the patient's WBC phagocytosis of Staphylococcus albicans obviously improve, serum preparin obviously elevated. Rabbits daily intramuscular injection of fisetin 8mg. 3 days after the continuous administration of the drug, the serum preparations in the solubilizing element is also significantly higher, fisetin to improve the body's immunity, the treatment of infectious diseases has an important significance.

2. Antibacterial effect

Fishwort extracted a yellow oil, on a variety of microorganisms (especially yeast and mold) have inhibitory effect on hemolytic streptococcus, staphylococcus aureus, influenza bacillus, cattacocci, pneumococcus has obvious inhibitory effect. It also has effect on Escherichia coli, dysentery bacillus, typhoid bacillus. The synthetic decanoylacetaldehyde sodium bisulfite adduct is called synthetic fisetin. Synthetic dodecanoyl acetaldehyde sodium bisulfite adduct is called new color fishin, in vitro and in vivo experiments on a variety of bacteria have more obvious inhibitory effect: on Staphylococcus aureus and penicillin-resistant MIC for 62.5 ~ 80mcg/ml, on the influenza bacillus for 1.25mg/ml. poupay's liquid medium for tuberculosis bacillus H37RY strain for 16mcg/ml. in the modified Suttons semi- solid medium it was 25mcg/ml, and its potassium salt was 12.5mcg/ml.The synthesized fisetin isonicotinone had a strong effect on Mycobacterium tuberculosis, with a MIC of 0.78-3.1mcg/ml on mice infected with TB. It can significantly prolong the survival time, 1mg per mouse intraperitoneal injection can extend the survival period of mice for 62 days, and the efficacy of feeding with food is better.

3. Antiviral effect

With human embryonic kidney primary monolayer epithelial cell tissue culture, fritillary decoction (1:10) on influenza Asian A Kyoko 68-1 strain has an inhibitory effect, and can delay the growth of orphan virus ECHO11. Zhu Yutong reported that several non-volatile oil fractions were obtained by ethanol extraction. Among them, fisetin Ⅲ had a preventive protective effect on influenza virus-infected mice, and had an insignificant effect on EMC and HSV Ⅱ. Derivatives of synthetic fisetin also had a strong antiviral effect. Fritillaria extract (4g raw/ml) showed significant prophylactic protection against recurrent influenza virus infected mice. And there was no significant protective effect on cerebral myocarditis virus and herpesvirus type II infection. It also proved that the anti-influenza virus component of Fritillariae chinensis was not in the volatile oil part, but in the non-volatile matter. In vitro experiments proved that the aqueous extract of Fritillaria can inhibit herpes simplex virus infections of type 1 and type 2, and the specific mechanism is to inhibit the activity of transcription factors required for viral replication.

4. Diuretic effect

The perfusion of toad kidney or frog web with Fritillaria extract can make capillary dilatation, increase blood flow and urine secretion, which has the effect of diuretic. Its effect may be caused by organic matter, potassium only play an additional role in increasing diuresis, direct action, can make the leptospirosis activity weakened - death - cleavage, but also can delay the onset of artificially infected guinea pigs with leptospirosis.

5. Anti-radiation effect

Fishsbill is the only one in the atomic bomb blast point can be tenacious regeneration of Chinese herbs. Fishweed has anti-radiation effect and enhance the role of the body's immune function, and without any toxic side effects, not only for aircrew, but also for those who are often close to the source of radiation, such as X-ray machines and computer operators, as well as the crowd often watching TV. The computer family must eat anti-radiation food.

6. and TMP pairing effect

and TMP pairing effect of the study, Zhang Yongheng reported, 0.75mg/ml fritillariae group and 0.75mg/ml fritillariae-7.5mg/ml TMP pairing group comparison (t=5.6) P<0.001, there is a very significant difference. 0.75mg/ml fritillariae-7.5 mg/ml TMP dosing group compared with 7.5 mg/ml TMP group (t=2.78) P<0.01, a significant difference. It indicated that the bacteriostatic effect of the combination of the two was superior to that of the single use, and there was a synergistic effect between the two.0.1ml/ml Fritillariae volatile oil had no bacteriostatic effect, and there was a highly significant antagonistic effect between 0.1ml/ml Fritillariae volatile oil-7.5mg/ml TMP mated group and 7.5mg/ml TMP group comparing (t=4.2) P<0.001, which indicated that the Fritillariae volatile oil could reduce the TMP The antimicrobial effect of TMP could be reduced by the volatile oil of Fritillariae vulgaris. However, there are exceptions, the combination of the two can enhance the effect of anti-paratyphoid fever and dysentery bacillus Sonnenheimer's dysentery, so in the affirmation of antagonism at the same time, should not be ignored for individual strains of bacteria have a synergistic effect.

7. Pathogenic microorganisms

Fish grass fresh juice of Staphylococcus aureus has an inhibitory effect, the effect is weakened after heating. In vitro test proved that the decoction of Corydalis has different inhibitory effect on many kinds of gram-positive and negative bacteria such as Staphylococcus aureus, Staphylococcus albicans, Streptococcus haemolyticus, Streptococcus pneumoniae, Streptococcus kathakii, diphtheria bacillus, Proteus mirabilis, shiga, shih, fuchsia, and Sungai dysentery bacillus, S. enterocolitica and so on. Fishweed ether extract in vitro against Mycobacterium tuberculosis has obvious inhibitory effect. Fishweed extracted from an oil, on a variety of microorganisms (especially on molds and yeast) have inhibitory effect on hemolytic streptococcus, staphylococcus aureus, influenza bacillus, katalog, pneumococcus has obvious inhibition of the use of fishweed; on Escherichia coli, dysentery, typhoid fever bacillus also have inhibitory effect. However, there are reports that the antibacterial effect of Fritillaria decoction is not very obvious.

Decanoylacetaldehyde, the main antimicrobial active ingredient of Fritillary, has obvious antimicrobial effect on a variety of bacteria, acid-resistant bacilli and fungi, etc., but the nature of this compound is not stable. Its sodium bisulfite adduct is stable and retain its antibacterial activity. China's synthetic decanoyl acetaldehyde sodium bisulfite adducts called synthetic fisetin, in vitro on a variety of Gram-positive and negative bacteria have obvious antibacterial effect to Staphylococcus aureus and its penicillin-resistant strains, Diplococcus pneumoniae, Streptococcus aureus, influenza bacillus is more sensitive to Kartococcus, typhoid fever bacillus followed by Escherichia coli, Pseudomonas aeruginosa and dysentery bacillus is not very sensitive to. For tuberculosis infection in mice, fisetin can significantly prolong its survival time. In addition, Candida albicans, Cryptococcus neoformans, sporococcus, Aspergillus, Mycobacterium smegmatis, ringworm red, ringworm, gypsum-like microsporidium, rust-colored microsporidium, ringworm sharks, etc., also have obvious inhibitory effect. Synthetic dodecanoyl acetaldehyde sodium bisulfite adduct is known as the new fisetin, in vitro on S. pneumoniae, S. typhi, S. aureus, E. coli and sporotrichum, etc. also have inhibitory effect. The synthetic pallidin isonicotinone has a strong inhibitory effect on Mycobacterium tuberculosis in vitro and in vivo.　

The decoction of Cichorium spp. has a strong inhibitory effect on Leptospira in vitro and in vivo. Direct action, can make leptospirosis activity weakening - death - cleavage, and can delay the artificial infection of leptospirosis guinea pig morbidity.　

With human embryonic kidney primary monolayer epithelial cell tissue culture, Fritillaria decoction (1:10) on the influenza Asian A Kyoko 68-1 strain has an inhibitory effect, and can delay the growth of orphan virus (ECHO11). It was proved that the anti-influenza viral components of Fritillariae vulgaris were in the non-volatile compounds. Fritillaria extract on influenza virus infection in mice has a significant preventive and protective effect, with the gavage and nasal drip route of administration have a significant effect; but the myocarditis virus and herpesvirus type II infections have no significant protective effect.

8. Role of the immune system

Fritillaria decoction in vitro can significantly promote human peripheral blood leukocytes phagocytosis of Staphylococcus aureus. Synthesized fritillarin can improve the phagocytosis of leukocytes in patients with chronic bronchitis, and increase the level of serum preparations of rabbits and patients.

9. Anti-tumor effect

The inhibitory effect of new fisetin on Ehrlich ascites carcinoma may be related to the increase of cAMP level in cancer cells. Experiments showed that when mice were injected with different doses of neohesperidin intraperitoneally at different times, the total number of cancer cells, the division index of cancer cells, and the amount of ascites decreased significantly, while the level of cAMP in the cancer cells increased.

10. Anti-inflammatory effect

Fishsbane decoction has significant anti-inflammatory effect on formaldehyde foot swelling in rats, and can also significantly inhibit the thermal denaturation of human γ-globulin in the presence of Cu++; Fishsbane can significantly inhibit the swelling of the mouse ear caused by crotch oil, xylene, the skin capillary permeability increases, the HCA-induced abdominal capillary dye exudation also has a significant inhibitory effect. Quercetin, quercetin and isoquercetin flavonoids contained in Fritillary also have significant anti-inflammatory effects, can significantly inhibit the early inflammation of capillary hyperactivity.

11. Other effects

Fishsbane oil can significantly antagonize the slow-reaction substances (SRS-A) on the isolated ileum of guinea pigs, can significantly inhibit the sensitized guinea pigs isolated ileum allergic contraction, antagonize histamine, acetylcholine on the contraction of guinea pigs ileum, and guinea pigs allergic asthma has a significant protective effect.　

Subcutaneous injection of aqueous solution of Corydalis has a mild sedative, anti-convulsive effect, can inhibit the spontaneous movement of mice, prolong the sleep time of cyclohexylbarbitone sodium, anti-convulsions caused by strychnine; 20-40mg/kg static injection in dogs can make its blood pressure drop 40-50mmHg (5.32-6.65kPa), and can inhibit the heart of isolated toads. Fritillaria decoction can inhibit plasma secretion and promote tissue regeneration; it also has analgesic and hemostatic effects. Intraperitoneal injection of ammonia spray-induced cough in mice has a cough suppressant effect, but no expectorant asthma.

Houttuynia cordata also has analgesic, antitussive, hemostatic, inhibit plasma secretion, promote tissue regeneration, wound healing to promote erythroderma, psoriasis, etc. Houttuynia cordata has the effect of stimulating the skin foam. Subcutaneous injection of aqueous solution of Houttuynia cordata also has a mild sedative, anti-convulsive effect, can inhibit the spontaneous activity of mice, prolong cyclohexabarbital sodium sleep time, to counteract the convulsions caused by strychnine, 20-40mg/kg pulse injection in dogs can make the blood pressure drop by 40-50mm Hg.

12.Toxicity

Toxicity of Houttuynia cordata is very low, subcutaneously injected in mice, the LD50 is 1.6±0.081g/kg, to the body weight of 17-20g mice intravenous injection of 1.5mg of fritillaries (equivalent to the human body about 200 times the injected dose), observed for 1 week without the phenomenon of death, and 90% of the body weight increase, the autopsy also did not see lesions occurring. Intravenous injection of 38-47mg/kg to dogs did not cause death, the dose increased to 61-64mg/kg can cause death, autopsy can be seen in the lungs with severe hemorrhage and thrombosis. Subcutaneous injection of 80～160mg/kg daily for 1mo, it can be seen that there is a large amount of salivation, and vomiting often occurs in the early stage, but there is no obvious effect on appetite, blood and liver and kidney functions. Gavage to the dog, daily 80 ~ 160mg / kg, for 1 month, can be seen in a large number of salivation phenomenon, early often appear vomiting, in addition to no abnormality.