Pupal temperature: the color of silkworm pupa may be affected by the ambient temperature. If the ambient temperature is high, the color of the pupa may be yellowish. If the ambient temperature is low, the color of the pupa may be blackish.

Aroma: Fresh yellow pupae have a fresher aroma with some sweet milky flavor. Fresh black silkworm pupae, on the other hand, have a stronger aroma of silkworm pupae, as they have more internal oils and are more fully cooked.