Effect of 1. on hypothalamus-pituitary-ovary axis: (1) Young rats were given this medicine orally on the 25th day after birth and HMG on the 28th day under the same conditions, and ovulation could be induced twice after 24h and 72h. In addition, taking pentobarbital sodium (SP) immediately after ovulation, which is equivalent to the critical period of releasing endogenous luteinizing hormone (LH), can affect the ovulation induced by pregnant horse serum hormone (PMS). HMC combined this drug with SP in the experiment, and the results showed that this drug may act on the center, especially the hypothalamus, and activate it. In another experiment, 30% female mice ovulated on the 3rd1day after birth, and injected with 15 unit of human menopausal gonadotropin (GNR4) intraperitoneally on the 28th day after birth, which made 65% female mice ovulate every time on the 29th day after birth, but pentobarbital sodium could not block this effect. GNR4 combined with this drug can make animals ovulate twice, each time on the 29th day after birth (60%) and each time on the 3rd1day (90%). Sodium pentobarbital has no effect on the first ovulation, but it can delay the second ovulation 1 day. The results showed that the drug directly or indirectly acted on hypothalamus, regulated pituitary-ovarian function and accelerated the ovulation process of neuroendocrine regulation. The drug has synergistic effect with nicotine, acting on nicotinic acetylcholine receptors in cerebral cortex and hypothalamus and estrogen receptors in cytoplasm to promote their synthesis. ⑵ This prescription may be a hormone activator, which can increase the secretion of luteinizing hormone and has synergistic effect with clomiphene citrate; It can increase the contents of estradiol (E2) and progesterone (P) in vivo. The effects of this medicine on serum FSH (follicle protectant), LH (luteinizing hormone), E2 (estradiol) and P (progesterone) in patients with functional uterine bleeding were determined by radioimmunoassay. After taking this medicine, the contents of the above four hormones in adolescent anovulatory patients, especially E2, increased, while in menopausal anovulatory patients, E2 and LH increased, while in fertile patients with ovulation, all four hormones decreased, and E2 and P decreased. Its main function may be to influence the release of pituitary FSH and LH through two-way regulation of E2 synthesis in vivo. ⑶ When the concentration of this prescription is 500μg/ml, the concentration of LH is 377.2% of that of the control group, and the concentration of FSH is 15 1.9%, but it has no effect on intracellular LH and FSH. ⑷ Danggui Shaoyao Powder can obviously stimulate the secretion of progesterone and estradiol-17β by mucosal cells, but it has no stimulating effect on the production of testosterone; It can also significantly stimulate progesterone, testosterone and estradiol-17β in follicular residues, and its effect on progesterone is stronger than that of monoene and estradiol-17β. It can regulate the concentration of luteal growth regulator C/ insulin-like growth factor-1 in rats. ⑸ In the rat pseudopregnancy model, this prescription can also increase the secretion rate of progesterone in ovarian venous plasma, and obviously increase the ratio of progesterone to 20α-OH progesterone. Another study showed that Danggui Shaoyao Powder had no obvious effect on the development, lactation ability, reproductive organs, sexual cycle and the ability of feeding young rats, but it could significantly increase the birth rate of young rats. The average productivity of 1-5 was 5 1% in the control group and 60% when drugs were mixed in the feed at the ratio of 0. 1%.
Foreign scholars have investigated the comprehensive effects of Danggui Shaoyao Powder on ovarian function: ① Rats were treated with PMS-hCG in vivo, and each drug extract was taken orally for 5 days, with a daily dose of 100-200μg/ rat, and the concentrations of progesterone (P), 17α-OHP and 20α-OHP in blood and ovary were determined. The values of serum P, 17α-OHP and P or 17α-OHP/20α-OHP increased in the group of Poria cocos+Paeonia lactiflora+Angelica sinensis, but the ratio of serum P/20α-OHP did not increase. In the ovary, the P/20α-OHP ratio increased when Poria cocos or Paeonia lactiflora was used alone, but decreased when Atractylodes lancea or Poria cocos+Atractylodes lancea was used alone. ⑵ Female rats were injected with 20IU pregnant horse serum gonadotropin (PMS) subcutaneously for 56 hours on 27th, then injected with human chorionic gonadotropin intraperitoneally, and the ovaries were removed 7 days later. In vitro culture of Danggui Shaoyao Powder and its compatible drugs showed that Poria cocos+Paeonia lactiflora+Alisma orientalis+Angelica sinensis, Poria cocos+Angelica sinensis, Angelica sinensis+Chuanxiong significantly increased progesterone secretion, and its level was higher than that of Danggui Shaoyao Powder alone, suggesting that this effect has a subtle compound effect.
4. Change hemorheology: This prescription has a good effect on pregnancy poisoning. The blood concentration and viscosity of pregnant women increase, and the fluidity decreases, resulting in microcirculation disturbance of placenta and organs. This recipe can obviously reduce the blood viscosity of pregnant rats, but has no obvious effect on erythrocyte deformability, hematocrit and fibrinogen concentration. Due to the decrease of blood viscosity, the circulating blood flow can be increased reversely, while the blood viscosity of most patients with severe pregnancy poisoning is increased. It can be seen that the above-mentioned effects of this prescription are beneficial to improve the circulating blood volume of placenta and kidney of patients, thus improving the symptoms of pregnancy poisoning. This prescription is also effective in treating functional uterine bleeding. Clinical research shows that the hemorheology of patients with this disease has obviously changed, which is characterized by prolonged erythrocyte electrophoresis time, increased plasma specific viscosity and decreased hematocrit, suggesting that the blood is in a cohesive state, reduced fluidity and reduced tangible components. After the treatment of this prescription, it can be seen that the erythrocyte electrophoresis time is obviously shortened, and the plasma specific viscosity and plasma osmotic pressure are obviously reduced. However, hematocrit and whole blood specific viscosity show China effect. Before treatment, these two indexes were higher in patients with blood stasis and qi stagnation, but the lowest in patients with deficiency of both qi and blood. After treatment, it was obviously decreased or increased, which made the patients with blood stasis and qi stagnation return to normal.
3. Effect on platelet coagulation and fibrinolysis system: This prescription has obvious inhibitory effect on platelet aggregation caused by collagen and ADP, and its effect is stronger than Guizhi Fuling Pill and weaker than Taohe Chengqi Decoction. Measured by active partial thromboplastin time (APTT) and prothrombin time (PT), the inhibitory effect of this prescription is stronger than Taohe Chengqi Decoction and Guizhi Fuling Pill, but the fibrinolytic activity measured by fibrin plate method and euglobulin lysis time is weaker than the above two methods. This prescription has the effects of inhibiting platelet aggregation, inhibiting coagulation and increasing hemorheology, in order to relieve the blood concentration, viscosity, coagulation and aggregation of patients with blood stasis syndrome, so as to obtain pharmacological basis for the curative effect of related diseases.
4. Improve microcirculation: The nailfold microcirculation of patients with functional uterine bleeding is abnormal, which is characterized by vague outline of loops, abnormal increase of loops, slow micro-blood flow, abnormal blood flow such as broken line, dotted line and flocculent flow, blood cell aggregation, congestion in loops, and decreased number. According to the classification, it can be seen that the appearance rate of fuzzy outline of loops of qi-blood deficiency type is obviously higher than that of blood stasis and qi stagnation type, while the number of loops of qi-blood deficiency type is obviously less than that of blood stasis and qi stagnation type. After the treatment of this prescription, it can be seen that the outline of capillary loops becomes clear from obscurity, the number of loops increases, the aggregation of blood cells decreases or disappears, the blood flow pattern changes from abnormal broken line flow, dotted line flow and flocculent flow to normal linear flow, the blood flow speed changes from slow to fast, and the blood stasis in loops decreases or disappears.
5. Effect on monoamine substances in brain: Mice were divided into two groups, one group was given orally and the other group was given repeatedly (twice a day for two weeks). Monoamines (norepinephrine, MHPG, dopamine, dobutamine, HVA, 5- hydroxytryptamine, 5-HIAA) in cerebral cortex, striatum and hippocampus were determined by HPLC. The results showed that the equivalent dose of each group was 50 mg. When the dose was 500mg/kg, DA and HVA in cerebral cortex increased, NE and MHPG in hippocampus decreased, and DA, DOPAC and HVA increased. When the dose was 50mg/kg, DA, DOPAC and 5-HIAA in cerebral cortex increased, NE and MHPG in striatum increased, and monoamines in hippocampus remained unchanged. At the dose of 500mg/kg, except HVA, the cerebral cortex increased, while norepinephrine, MHPG, dopamine and serotonin in striatum increased, while monoamine neurotransmitters in hippocampus did not change.
6. Others: This prescription has obvious anti-inflammatory effect, which can significantly inhibit the inflammation and exudation of rat croton oil granulation sac, but has no obvious effect on the tissue proliferation of cotton ball granuloma. It can improve the immunosuppression of prednisolone and promote the mitogenic activity of PHA. In addition, this medicine can also regulate the function of autonomic nerve.