1. Pairing conditions, first of all, make sure that the zebrafish pairing conditions are correct, usually use the same age, similar size fish for pairing. Before pairing, you should make sure the fish are in healthy condition and place them in a new water environment.
2. Time control, the time for collecting eggs needs to be decided according to different experimental purposes. If a large number of eggs are required, the males and females can be paired together and separated at night. The next morning, the eggs can be collected from the females. If a small number of eggs are required, the males and females can be placed in separate tanks and paired after about 14 hours.
3. Eggs can be collected using either a glass pipette or a pipette, depending on the specific needs of the experiment. If a large number of eggs are needed, the eggs will be sucked out using a pipette; if a small number of eggs are needed, a small glass maker will be used to remove the eggs and place them into a petri dish. In summary, it is very important to control the pairing conditions and egg collection time of zebrafish, which will directly affect the accuracy and reliability of the experimental results. The success of zebrafish experiments can be ensured by reasonable pairing and adopting appropriate ways of collecting eggs.