① Beet: During the period of 10 ~ 12d after inoculation, chlorosis spots appeared on inoculated leaves, followed by systemic yellowing and necrosis along the veins.
(2) Spinach and big-fruit beet: The inoculated leaves appear chlorosis spots, and then infected by the system, the veins turn yellow, the leaves are deformed and wilted.
(3) Chenopodium album and amaranth: 5 ~ 6 days after inoculation, the inoculated leaves appear yellowing or local necrosis, and there is no systematic infection.
④ Prunus armeniaca: 7-8 days after inoculation, the inoculated leaves showed yellow spots or bright yellow spots, and later developed into necrotic spots and concentric ring spots.
Hosts of Fusarium wilt: Chenopodium album, Atriplex angustifolia and Apricot.
In vitro stability:
① Passivation temperature: 60 ~ 65℃ (China isolate) and 65 ~ 70℃ (Japanese isolate).
② Dilution endpoints: 10-4 ~ 10-3 (China isolate) and 10-4 (Japanese isolate).
③ Survival period in vitro: 5 ~ 6 days (China strain), 5 days at 20℃ and 8 days at 4℃ (Japanese strain).
Serological detection: the virus has high immunogenicity, and there is no serological difference between different strains. It is serologically related to the same beet soil-borne mosaic virus, but not to fungal-borne viruses (soil-borne wheat mosaic virus, potato broom top virus, peanut witches' broom virus), tobacco mosaic virus and tobacco brittle fracture virus. Antiserum was prepared by virus purification and prokaryotic expression of coat protein products, and a detection kit was developed for virus detection. In addition, monoclonal antibodies were prepared.
Electron microscopic observation: virus particles scattered in cytoplasm or gathered in angular layers, rough endoplasmic reticulum increased, and distributed irregularly in mesophyll cells infected by the system. The partially purify virus or diseased tissue juice can be observe and detected by an electron microscope.
Quarantine risk assessment: The pathogen of the disease is beet necrotic yellow vein virus, which can spread continuously through polymyxin beet and seriously harm beet. At the time of risk assessment, Ji Liang is a high-risk pest with a risk value of 9. It occurs in China, and it belongs to non-quarantine pests because there is no artificial transmission route. However, in view of the harmfulness of agricultural production, it should be included in the focus of domestic prevention and control.
Distribution: China (Inner Mongolia, Xinjiang, Heilongjiang, Jilin, Shanxi, Ningxia and Gansu), Italy, Britain, France, Germany, Sweden, Netherlands, Belgium, Austria, Switzerland, Greece, Romania, Bulgaria, Spain, Turkey, Yugoslavia, Hungary, Czech Republic, Slovakia, Poland, Russia, Kazakhstan and Kyrgyzstan.
Quarantine countries: Bulgaria, Poland, Netherlands, Croatia, European Union, European and Mediterranean Plant Protection Organization, Slovenia, Tunisia, Turkey and Hungary.