Isothermal amplification method is a new molecular biological detection method, which can complete the cyclic amplification of the target sequence without denaturation and annealing steps in PCR, greatly shortening the time. The whole amplification reaction time is generally less than 60 min, while the reaction time of conventional PCR method generally takes more than 2 h. Isothermal amplification kit is suitable for on-site detection, with high sensitivity, which can greatly improve the feasibility of prevention and control of classical swine fever.
To reduce the number of off-site personnel and vehicles entering the pig farm, it is necessary to thoroughly disinfect the personnel and vehicles before entering the pig farm, implement all-in and all-out feeding management for pigs, isolate the newly introduced pigs, and declare quarantine according to regulations.
Precautions for prevention and control of African swine fever
Farms should adhere to the all-in, all-out, self-propagating and self-supporting breeding mode, and build perfect blockade and isolation measures to avoid direct contact between pigs and wild boars. In the process of pig transportation, farms should try not to introduce breeding pigs across provinces. When it is necessary to introduce, strict quarantine and epidemic detection must be carried out, and the landing report system and isolated observation system must be strictly implemented.
If a farm finds a suspected African swine fever epidemic, it shall deal with it in strict accordance with the emergency plan for African swine fever epidemic and take emergency measures quickly to prevent the epidemic from spreading and spreading.