Yeast two-hybrid technology

Advantages:

1. Large scale screening,

2. Simple and intuitive operation (fluorescence can be used as reporter gene, and amino acid deficiency plate screening can also be used, etc.)

Disadvantages:

1. The traditional yeast two-hybrid technique (it seems to be published in the journal nature in 1989, you can check it out, but you can't remember it exactly) can't study the interaction of membrane proteins. The new technology derived from this is difficult to study the interaction of membrane proteins.

2。 The interaction between the target proteins studied by yeast two-hybrid technology needs to occur in the yeast nucleus, so a new problem arises: if yeast two-hybrid technology shows that two proteins interact (that is, they do interact in the yeast nucleus), but the two interacting proteins are located in different organelles in the original host (that is, they can't appear in the same position), then false positives will occur, and there are many examples.

Despite the above shortcomings, yeast two-hybrid technology has greatly promoted the study of protein interaction.

immune * * * precipitation technology:

it can make up for the shortcomings of the above yeast two-hybrid technology, but it has no advantages of the above yeast two-hybrid technology.

the combination of the two technologies can complement each other's shortcomings and superimpose their advantages.