Alias Ge Ye rhubarb, rhubarb
source
Source of medicinal materials: roots and rhizomes of dicotyledonous plants of Polygonaceae.
Latin plant and animal mineral name: Alexander rhubarb.
pharmacological action
The water extract of this product has obvious inhibitory effect on human platelet aggregation induced by collagen, and its IC50 is 1.3mg/ml, which is lower than that of rheum tanguticum (0.68mg/ml) and higher than that of rheum palmatum (2.73 mg/ml) [1 2]. This product also contains anthraquinone components emodin, emodin methyl ether and chrysophanol [3], 1. Mechanism of antipyretic, analgesic and anti-inflammatory action 1. 1. Cold soaking agent of raw rhubarb: raw rhubarb is crushed into small pieces, soaked in cold water twice, each time for 24 hours, and the combined soaking agents are concentrated to 80% below 40℃. Rhubarb decoction 10'': small pieces of raw rhubarb are soaked in water for 24 hours and then decocted for 10 minute, then the decoction is poured out and decocted in water for 10 minute, the decoctions are combined and concentrated in water bath to form 80% decoction. Radix et Rhizoma Rhei decoction 30'': The method is the same as (2), decocted twice for 30 minutes each time, and concentrated into 80% decoction in water bath. Cooked rhubarb decoction: 500g of raw rhubarb is sliced, 250g of yellow wine is added, steamed 12 hours, dried, soaked in water for 24 hours, sliced and fried twice, each time for 30 minutes, and the two decoctions are concentrated into 80% decoction in water bath. Imitating Max's method, five rats in each group were injected with 25g/kg of the above four rhubarb extracts, and the same amount of distilled water was used as control. Immediately after administration, sc 15% fresh yeast saline suspension was 2ml/ 100g, and the changes of body temperature were observed within 7 hours. The results showed that the extracts prepared by different methods had obvious antipyretic effects, but there was no significant difference between the groups. Analgesic effect: the experiment was carried out by twisting reaction method. Twenty mice in each group were given 25g/kg of the above four rhubarb extracts and irradiated with distilled water. After 1 hour, ip 1% acetic acid was 0. 1 ml/ 10g, and the number of writhing reactions was observed within 20 minutes. The results showed that all four kinds of rhubarb extracts had certain analgesic effects, but there was no significant difference in analgesic effects among the four kinds of rhubarb extracts. Recently, it has been reported that rhubarb extract can inhibit the activity of sodium pump in normal human erythrocytes and the active transport of Na+ and K+ ions, and this effect is enhanced with the increase of rhubarb concentration. Rhubarb inhibits Na+ and K+-ATPase activities, thus reducing ATP decomposition and productivity, which is one of the antipyretic mechanisms of Rhubarb.
The effect of 1.2 on cAMP, a central regulator of body temperature; Radix et Rhizoma Rhei decoction pieces were made into 30% decoction, and the decoction was given orally at a weight of 5g/kg. Fever model was made by pneumococcus (type ⅱ) provided by the Institute for Biological Products Inspection of the Ministry of Health, and infectious fever animal model was made. Then the content of cAMP in the perfusion fluid of the third ventricle of rabbits was determined by radioimmunoassay. Results: Rhubarb can reduce the cAMP level of rabbits with infectious fever: Seven rabbits were perfused into ventricles three times within 24 hours, namely, normal body temperature (a), fever peak (b) and after rhubarb was given to reduce fever? Fever peak (b) and after giving rhubarb to reduce fever? The contents of cAMP in perfusates A, B and C3 were detected respectively. The results are as follows: A3.37pmol/ml, B6.48pmol/ml, C3.07pmol/ml. It can be seen that the content of cAMP at point C after rhubarb administration is significantly lower than that without rhubarb administration. There was significant difference in cAMP content between group C and group B (P
The cooling effect of rhubarb 1.3 and its effect on PGE in central nervous system The effect of rhubarb 1.3 1 on the body temperature of rabbits with infectious fever was studied. Lan Tu, a healthy adult, weighed 2.2kg-3.5kg. The fever model was prepared as above. Fever animals were divided into experimental group and control group, with 65438 07 animals in each group. The experimental group was given rhubarb decoction (5g crude drug /kg) at the peak of fever, while the control group was given the same amount of tap water. After administration, the anal temperature was measured 65438 0 times every 4 hours for 3 consecutive times, and the peak of fever was compared with the anal temperature after cooling. Results rhubarb can reduce the rectal temperature of rabbits with infectious fever: rhubarb decoction (8 rabbits) and white water (8 rabbits) were given to rabbits with fever caused by pneumococcal infection, and the reduction range of rhubarb group was significantly higher than that of drinking water group (X s1.25 0.42), P < 0.0 1.42. Measurement1. The rectal temperature before and after administration was compared. The average anal temperature before and after rhubarb administration was 39.5 0.65438 0.8℃ and 39.7 0.43℃, respectively, p > 0.05；. 0.0 1, human yellow has no obvious effect on the body temperature of normal rabbits.
The effect of 1.3.2 on PGE in central nervous system The experimental animals and model preparation methods are the same as above. The content of perfusion fluid in the third ventricle was determined by radioimmunoassay established by Li et al. Within 24 hours, five rabbits were perfused intraventricular three times, namely, when the body temperature was normal (a), when the fever peaked (b) and after rhubarb was given to reduce the fever? The contents of PGE in perfusates A, B and C3 were determined respectively. The results are as follows: A: 2.2 1.5 ng/ml, 4.9 2.2 ng/ml, C: 1.5 1.2 ng/ml. The content of PGE in perfusate of group C after increasing jaundice was significantly lower than that of group C without increasing jaundice, and there was significant difference between group C and group B (P
1.4 Effects of rhubarb on endotoxin-induced fever in rabbits and the contents of cAMP and cGWP in plasma in recent years, studies have shown that when endotoxin causes fever in animals, the content of cAMP in plasma and cerebrospinal fluid increases obviously, but the effect on cGMP content has not been reported. Therefore, rabbits with big ears were divided into two groups to enter the experiment: the drug group was given rhubarb decoction for a certain period of time, and then a certain amount of Escherichia coli endotoxin was injected to cause fever. Anal temperature was measured 6 times in 6 hours. Blood was collected from ear artery 65 438 0.5-2 hours before and after endotoxin injection, and the contents of cAMP and cGMP in plasma were determined by competitive protein binding method and radioimmunoassay respectively. The results showed that the average peak fever T (40,15 0.21℃) and the average body temperature response index tri6 (10.97+1.20cm2) in the treatment group. All of them were significantly lower than the control value (40.865438 0.23℃ accounted for 20.96 l.69cm2). Plasma of drug group. The average content of cAMP (43.75 7.01pmol/ml) was 43.75 7.01and 47.76 9.1respectively. 0.05)。 However, the average plasma cGMP content (pmol/ml) in the medication group had no significant difference before and after heating (365,438+0.39 4.65,27.19 6.15 respectively), while the average plasma CGMP content in the control group decreased significantly after heating (30.7/kloc-0 respectively). From this, we can calculate the changes of the plasma cAMP/cowtP ratio before and after fever, but there is no obvious change in the medication group (1.43 0.36 and 1.84+0.57). However, the fever in the control group increased significantly (65438 0.44 0.36 and 2.68 0.62). The above results show that rhubarb has obvious inhibitory effect on endotoxin-induced fever in rabbits. Rhubarb can inhibit the increase of cAMP and the decrease of cGMP in rabbit plasma.
1.5 Anti-inflammatory Mechanism of Raw Rhubarb1.5./Effect of kloc-0/on Egg White Foot Swelling A. Egg White Foot Swelling Experiment of Normal Rats: Twenty-eight male and female rats weighing 152-209g were divided into four groups, and they were given 5 ml normal saline and 20 g rhubarb decoction respectively. The percentage of foot swelling per hour before and after treatment was obtained by measuring the volume of fresh egg white (0. 1 ml/egg) in sole sc, and the hourly urine volume and diarrhea time were recorded. Results Both 20g/kg and 40g/kg rhubarb decoction had significant anti-egg white-induced paw edema (P values were respectively
Preventive effect of 1.5.2 on formaldehyde-induced paw swelling in rats Thirty rats, weighing 176-240g, half male and half female, were divided into three groups. 1 group was given rhubarb decoction 20g/kg, and 1 group was given sodium salicylate sc25%mg/kg intraperitoneally. Results The control group was 42.4 7.2, rhubarb group was 65,438 09.5 2.7 (P < 0.05), and sodium salicylate group was 26.3±5.3(P = 0.05)65) 65,438 0.5.3. After operation, each group was given saline 5 ml and rhubarb decoction 10g/kg respectively, while the other group was given hydrocortisone ip30mg/kg, once a day/kloc-0. On the eighth day, cotton granuloma was taken out, dried and weighed. Results: The dry weight of cotton balls in the control group was 98.4 4.9 mg, and that in the rhubarb group was 765,438 0.4 mg. 0.0 1), and the hydrogen test group was 48.51.5 mg (p < 0.001).
Effect of 1.5.4 on the content of ascorbic acid in adrenal gland of rats. Rats in the two groups were given 5 ml normal saline and 40g/kg rhubarb decoction respectively. Two hours later, the animals were killed, the adrenal glands were taken out, and the content of ascorbic acid was determined by Roe method. Results The control group contained 1 1.5 1.5 mg ascorbic acid per 1g adrenal tissue, which was a large amount.