Role of guarana
Antibacterial and anticancer effects. Juniperus communis has certain inhibitory effects in vitro on Escherichia coli, Sonnet's dysentery bacillus, and Proteus mirabilis. The water infusion (1:2) also has different degrees of inhibitory effects on certain skin fungi in vitro. In in vitro tests, a decoction of whole Juniperus communis (20% decoction) had a lethal effect on ascites cancer cells. It has also been suggested that both rarouni bark and rarouni kernel are effective, but the former is better. The seed husk and fatty oil are not effective. Alcohol and ether extracts are also effective. 60% alcohol extract has the best effect in vitro. However, in animal tests, its action is less significant and unstable; in vivo, the effect on sarcoma is a little stronger than on ascites cancer cells.
Expectorant effect
Animal experiments show that the total amino acids fractionated from the skin of melon atrophy have a good expectorant effect.
Laxative effect
Gua shui bian contains diarrhea-causing substances and has a laxative effect. The bark of Psidium guajava is weak; the kernel is strong; and the cream of Psidium guajava acts as a moderator.
Actions on the cardiovascular system
Concentrate of decoction and alcohol precipitation of the bark of Psidium guajava (35%) seeds (65%), and injection made from the part of Psidium guajava bark ointment exchanged by cationic resin (referred to as Psidium guajava injection), both have coronary dilatation effects on guinea-pig isolated hearts, with the latter being more pronounced. A dose of 2.5 or 50 mg of the raw drug per 1 ml of infusion increased coronary flow by 55% or 71%, respectively. Similar results were obtained in the isolated heart-free test. Psidium guajava injection has obvious protective effect on acute myocardial ischemia induced by pituitary post-temporal hormone in rats; and it can obviously improve the tolerance of mice to normobaric and low-pressure hypoxia; it can also improve the survival rate in low-pressure hypoxia in mice given isoproterenol by subcutaneous injection in advance, but it doesn't prolong the sleeping time of sodium secobarbital to mice, suggesting that its effect of improving the tolerance of hypoxia is not related to the central nervous system. The intensity of the coronary expansion effect of different parts of Psidium guajava was: Psidium guajava peel> Psidium guajava cream> Psidium guajava seeds> Psidium guajava kernel> Psidium guajava husk.
Antibacterial effect
1:5-1:1 Psidium guajava decoction or infusion, in vitro on Escherichia coli and other gram-negative intestinal pathogens have an inhibitory effect; and staphylococcus, S. pneumoniae, Streptococcus a haemolytic streptococcus, influenza bacillus, small bacillus of Audu ounce and astro nucella, etc., but also have a certain inhibitory effect.
Anti-cancer effect
1:5 decoction of Psidium guajava in vitro (slide method) kills ascites cancer cells in mice. The in vitro anticancer effect of Psidium guajava peel was better than that of Psidium guajava kernel and was strongest with 60% ethanol extract. The off-white amorphous powder obtained from the ether extract of Psidium guajava also had in vitro anticancer effects, and animal tests showed that Psidium guajava had a certain inhibitory effect on sarcoma. The effect on ascites carcinoma was not obvious. It has also been reported that the preparation of Psidium guajava was ineffective against transplanted U-14 and S-180 in mice.
Anti-ulcer effect
The fruit of Piper betle was extracted three times with 50% ethanol at 90°C, and then vacuum-dried to obtain a black-brown powder (TKE). In the experiment of pylorus ligation of rats, TKE500 and 1000mg/kg significantly reduce the concentration of gastric acid secretion and gastric acid in rats, and 100mg/lg TKE increases the above indexes. 100, 500, 1000mg/kg TKE has an inhibitory effect on the ligation of pylorus induced ulcers, the inhibitory rate of 44.4%, 68.2%, 84.1%, respectively. The same dose of TKE reduced the gastric injury induced by water immersion pressure method in rats by 16.5%, 51% and 66.9%, respectively. The difference was not significant for histamine-induced gastric mucosal injury, although it was attenuated.TKE at 500mg/kg and 1000mg/kg protected against 5-hydroxytryptamine-induced gastric mucosal injury, with the inhibition rates of 59.1% and 63.6%, respectively. With prophylactic administration, TKE (500, 1000 mg/kg) inhibited the gastric mucosal injury induced by ethanol hydrochloride by 80.9% and 94.8%, respectively. Some necrotizing agents such as 1 ml of 0.6 mol HCI and 0.2 mol NaOH or 35% NaCl can cause severe gastric mucosal injury, which can be attenuated by preadministration of TKE. In addition, TKE healed gastric ulcers caused by acetic acid. In in vitro experiments, 2.5×10(-3) g/kg and 1.0×10(-2) g/kg of TKE had significant relaxing effects on acetylcholine-induced ileal contractions in mice. It indicated that the higher dose of TKE had inhibited gastric acid secretion and anti-ulcer effect, and TKE reduced the ulcer induced by acetic acid, suggesting that TKE may have a therapeutic effect on chronic ulcers.
Delaying the aging effect
2.5% guarana acid alcohol extract composition, observed with the effect on the fertility of Drosophila melanogaster, in 10-15 days the number of offspring of Drosophila control group was 65.8 ± 13.0, while the guarana group was 130.2 ± 12.6 (compared with the peak of the control group p