That is, 1 6×Loading dye was added to 5 parts of DNA solution. For example, 2ul 6× loaded dye is added to the 10ul DNA ladder.

The loading solution of 6× loading dye contains 10mM Tris-HCl(PH8.0), 1mM EDTA, 2.5% bromophenol blue and 4% sucrose.

Recommended gel concentration or sample loading: 2.0 ~ 2.5% agarose gel (final concentration of bromoethane: 0.5 ug/ml); 5 ~ 10ul/ glue hole.

Repeated freezing and thawing should be avoided during storage or use of DNA markers; In the process of long-term freezing, DNA concentration gradient may be formed, so it should be shaken evenly and centrifuged briefly after thawing.

Extended data

1 and the function of loading buffer:

The indicators in (1) are bromophenol blue and xylene blue, which show the progress of electrophoresis so that we can stop electrophoresis in time.

(2) Glycerol can increase the sample density, making the sample density greater than TAE, thus settling into the sample hole and preventing the sample from floating out of the sample hole.

2. SDS is adde to some buffers, which is commonly said. SDS mainly promotes the denaturation of polymerase, because the unfinished polymerase will combine with DNA double strand, which will affect its migration rate. The lysate after cell lysis is heated under the load of 65438 000℃ to stop the enzymatic reaction and prevent the extracted protein from degrading.

Baidu encyclopedia-load buffer

Baidu encyclopedia-100bp DNA ladder