1. Agrocybe aegerita is a mesophilic fungus. The temperature range of mycelium growth is 5 ~ 35℃, the optimum temperature range is 2 1 ~ 28℃, and the optimum temperature range is 25 ~ 27℃. It grows faster at 30℃, but slightly weaker. When the temperature is lower than 20℃, the growth rate slows down. Mycelium has strong adaptability to low temperature and high temperature, and it can still maintain vitality for 5 days at-65438 04℃ and 4 days at -40℃. The primordium differentiation temperature is 10 ~ 16℃, and the fruiting body development temperature is 13 ~ 25℃, which is a constant temperature fruiting type and does not need temperature difference stimulation. However, the temperature difference can promote the differentiation and growth of fruiting bodies to a certain extent, and can obviously increase the yield when the temperature drops suddenly in summer and autumn. When the temperature difference between day and night is above 65438 00℃, it only takes 2 ~ 3 days from budding to harvesting. When the temperature difference between day and night is less than 5℃, it takes 4 ~ 5 days from budding to harvesting. The differentiated primordia had poor low temperature tolerance, and the immature fruiting bodies died after 3 days at 4 ~ 6℃. At present, the fruiting body temperature of the selected strains used in production is different, and the optimum temperature of the fruiting body of the strain with low medium temperature is13 ~18℃; The optimum temperature of this strain is 16 ~ 28℃, and the optimum temperature is 24℃.

2. Pleurotus eryngii is a hygrophilic fungus. In the mycelium growth stage, the medium can grow normally when the water content is between 46% and 80%, and 64% to 67% is the most suitable. The growth of fruiting body needs high environmental humidity, and 85% ~ 90% is the most suitable. Excessive environmental humidity is not conducive to the development of fruiting bodies.

3. The mycelium growth stage does not need light, strong light will inhibit the mycelium growth, but it will not form fruiting bodies in complete darkness. Therefore, this characteristic can be used to avoid mushroom growth at the same time. The formation of primordia and the normal development of fruiting bodies need 500 ~ 1000 lux of light, but they can still grow normally under the weak light of 25 ~ 300 lux. Poplar mushroom has obvious phototropism. In cultivation, as in Flammulina velutipes cultivation, high-quality products with long stems and small lids can be obtained by shading with paper tubes.

4. Agrocybe aegerita is an aerobic bacterium, which needs sufficient oxygen supply in the growth stage, and abnormal mushrooms are easy to appear when oxygen is insufficient. The concentration of carbon dioxide should be controlled at 0. 15% ~ 0.2% in the mycelium growth stage, 0.05% ~ 0.08% in the primordium differentiation stage and 0.03% ~ 0.05% in the fruiting body growth stage. If the concentration exceeds the above, it should be ventilated in time. However, during the fruiting period, there is a little high carbon dioxide on the surface of the material, which is beneficial to the elongation of the stem, which is the same as that in the cultivation of Flammulina velutipes.

5. The pH value of Agrocybe aegerita likes to grow in a slightly acidic environment. The pH value of mycelium growth ranges from 4 to 6.5, but it also grows well in weak alkaline environment. In the fruiting stage, the pH value on the surface and inside of the culture medium changed little, ranging from 5.77 to 5.87, indicating that the mycelium produced little organic acid during metabolism, and it was generally unnecessary to adjust the pH value when fruiting.