1. Nutrient broth medium

Beef extract 0.3g

Peptone 1.0g

NaCl 0.5g

pH 7.7.2

Add water to the beaker, weigh beef extract, peptone and NaCl, heat and dissolve, adjust the pH to 7.7.2. Install, add cotton plugs, and sterilize with high-pressure steam. It is often used to culture bacteria.

2. Nutrient agar medium

Add 20 grams of agar to the nutrient broth medium. into. Commonly used to culture bacteria.

3. Meat peptone liquid medium

500 grams of beef

10 grams of peptone

NaCl 5 Gram

pH 7.7.2

Take 500 grams of fresh beef, remove the fat and tendons, mince or chop it, add 1000 ml of water to soak, and place it at 15°C for 12 hours Or leave it at 50℃ for half an hour. Filter the gravy with gauze to make up for water loss. Add peptone and salt to the gravy. Heat the gravy, add soda (sodium carbonate) until it turns red, adjust the pH value, aliquot and sterilize with high-pressure steam Bacteria.

Use cotton to filter the agglomerated protein from the above sterilized culture medium to make a liquid culture medium. If you need to make a solid culture medium, add 2 grams of agar to every 100 ml of liquid. , after heating and melting, aliquot and sterilize by high-pressure steam again. Commonly used to culture bacteria.

4. Gao's No. 1 medium (starch agar medium)

Soluble starch 2g

K2HPO4 0.05g

MgSO4·7H2O 0.05g

KNO3 0.1g

NaCl 0.05g

FeSO4 0.001g

Agar 2g

Water 100ml

pH 7.7.4

Add 95ml of water to the beaker and heat When it boils, take 2 grams of soluble powder, mix it into a paste with 5 ml of water, pour it into the boiling water and mix well. Then weigh other drugs and add them into the beaker one after another (after one drug is dissolved, add the second drug ). After all the drugs are dissolved, stop heating, make up for water loss, and adjust the pH value to 7.7.4. After dispensing, sterilize with high-pressure steam. This culture medium is often used to culture actinomycetes.

5 .Potato sucrose medium

200 grams of potato

10 grams of sucrose

20 grams of agar

1000 ml of water

Weigh 200 grams of potato slices, add 1000 ml of water, boil for half an hour, filter with gauze to make up for water loss. Add 10 grams of sucrose and 20 grams of agar to the above filtered juice, and heat The agar is melted. After packaging, it is sterilized by high-pressure steam. It is often used to cultivate yeast.

6. Wort medium

The wort with hops will be purchased from the brewery. , put into an Erlenmeyer flask, stoppered and sterilized by high-pressure steam. Commonly used for cultivating yeast.

7. Chassar's medium

NaNO3 2 grams

K2HPO4 1g

KCl 0.5g

MgSO4 0.5g

FeSO4 0.01g

Sucrose 30g

15 to 20 grams of agar

1000 ml of water

Natural pH

Aliquot, add cotton plugs, and sterilize with high-pressure steam. Commonly used to culture mold.

8. Bean sprout juice glucose (or sucrose) medium

100 grams of soybean sprouts

50 grams of glucose

15 grams of agar

1000 ml of water

Weigh 100 grams of fresh soybean sprouts, put it into a beaker, add 1000 ml of water, boil for 30 minutes, filter with gauze to make up for water loss. Add glucose (or sucrose ) 50 grams, 15 grams agar, heat until melted to make up for water loss. Pack, add cotton plugs, and sterilize with high-pressure steam. Commonly used to culture mold.

9. Eosin-methylene blue medium (E , M, B culture medium)

Lactose 10 grams

p>Peptone 5g

NaCl 5g

K2HPO4 2g

20 ml of 2% eosin aqueous solution

0.35% methylene blue 20 ml of aqueous solution

20 g of agar

1000 ml of water

pH 7.2