The artificial propagation method of loach is the same as that of domestic fish. The types and dosage of oxytocin used are: carp pituitary 0.5 ~ 1, frog pituitary 2 ~ 3, chorionic gonadotropin 100 IU ~ 150 IU, or the like. Microgram ~ 10 microgram. The dosage of male loach is halved. The injection amount of solvent was 0. 1ml ~ 1.2ml, and it was injected with a syringe of 1ml and a No.4 needle. The injection site is between the ventral line, pectoral fin and ventral fin, and it can also be injected into the dorsal muscle. Because loach likes to drill, it can be wrapped with wet gauze when injecting (exposing the injection site). After injection, it is put into the spawning pond, and then the work is the same as natural reproduction. When the water temperature is 20℃ ~ 25℃, 10 hour ~ 15 hour later, you can estrus and lay eggs.
If artificial insemination is used. Male and female loaches injected with oxytocin can be temporarily kept in ponds or cages respectively. When the water temperature is 18℃ ~ 25℃, after 10 hour ~ 15 hour, female loach can be artificially inseminated by dry method, and the operation methods and precautions are the same as those of domestic fish. Eggs go to the nest immediately after artificial insemination. Methods: Take a bucket of clean water, put the fish nest flat on the bottom of the bucket, then one person gently shakes the fish nest while stirring the water body, and the other person slowly pours the eggs into the bucket to make the eggs nest evenly. After nesting, transfer to an incubator for incubation.
The fertilized eggs of Misgurnus anguillicaudatus can hatch normally in the water temperature range of 20℃ ~ 30℃, and the fry can usually hatch in about 2 days. The best hatching effect is to use a small container of still water and change the water frequently (twice a day), and it is appropriate to hatch 400 ~ 600 capsules per liter of water. Avoid electric shock when hatching.
The newly hatched loach seedlings can't move freely, because their heads are attached to fish nests or other objects and feed on egg yolks. The fry hatched in the pond are generally cultured in the original pond, with the water depth of 20 cm ~30 cm and the density of 500 ~ 1500 per square meter. When the density is too high, some fry should be taken out and cultivated in another pond.
Loach seedlings began to feed after 3 days of incubation. At this time, the cooked and ground egg yolk or fish meal suspension should be fed several times a day, and the feeding amount should be limited to 1 hour. After 2 ~3 days, replace the Daphnia, rotifer, crushed earthworm or silkworm chrysalis. After about 10 days of cultivation, when the fry grow to 1 cm, they can be transferred to the fish pond for feeding; If the density is low, it can be raised in a pond. After about 1 month, when the fry grow to 3 cm, they can be transferred to fish ponds, or directly put into rice fields, ponds or pits for adult fish culture.
The area of seed pool should be 30 ~50 square meters. Before sowing, the pond should be cleaned and disinfected, and basic fertilizer should be applied to cultivate bait organisms. The sowing amount is about 30 grams per square meter (800 ~ 1000). Within half a month after the fingerlings enter the pond, powder compound feed is fed twice a day, and the dosage is 3%~5% of the fingerlings' weight. In summer, the temperature of shallow water in fish ponds is high, so we should always pay attention to the change of water quality and change the water frequently. When the fish grows to about 10 cm, it enters the feeding stage of adult fish.