Cordyceps militaris, also known as Cordyceps militaris, is a different species from Cordyceps militaris and is used as a substitute for Cordyceps militaris.

In recent years, Cordyceps militaris has been more and more loved by people.

So, how to plant Cordyceps militaris? Let's take a look at the artificial cultivation techniques of Cordyceps militaris.

Artificial cultivation technology of Cordyceps militaris 1, and selection of high-quality strains are suggested to go to regular universities, scientific research institutes and professional companies related to Cordyceps militaris research to buy the first generation of Cordyceps militaris mother seeds, or buy the growing fruiting bodies of Cordyceps militaris and separate them into test-tube mother seeds by themselves.

Stored in the refrigerator at 0 ~ 4℃ for no more than 3 months, the test tube seeds must be guaranteed within 3 generations. Before mass production, it is very important to do a weeding test to ensure the normal strains.

2. Select the optimized medium 2. 1 mother medium lactose 15g/L, sucrose 20g/L, peptone 15g/L, beef paste, 5g/L, potassium dihydrogen phosphate 2g/L, magnesium sulfate 1g/L, VB/L.

2.2 liquid culture medium potato 100g/L, sucrose 20g/L, peptone 15g/L, beef paste 5g/L, potassium dihydrogen phosphate 2g/L, magnesium sulfate 1g/L, VB 120mg, VB2/kloc.

2.3 solid medium: 20g of rice and 25mL； of water; Rice 13.4g, wheat 6.6g, nutrient solution 25ml [4]; Wheat 25g, nutrient solution 33mL；; Adopt 500mL can bottle; Wheat 30g, nutrient solution 51ml; 750mL can bottle is used.

The optimum formula of nutrient solution was sucrose 2%, peptone 2%, potassium dihydrogen phosphate 0. 1% and magnesium sulfate 0.2%.

Under normal circumstances, the biological efficiency reaches more than 65%, which is regarded as normal weeding.

The optimized mother culture medium can meet the nutritional growth of Cordyceps militaris strains to the greatest extent, and the mycelium is pure white and thick, which can maintain the vitality of the strains; The optimized liquid strain is clear and transparent, such as uniform and stable millet, which does not precipitate for three minutes and has a strong aromatic flavor; The optimized solid cultivation species can ensure the normal growth of fruiting bodies and realize small input and large output.

3. After conventional sterilization, when the temperature drops to 28℃, inoculate 5mL of liquid strain into the sterile operation table, and place it in the spawning room at room temperature of 25℃ for 4 ~ 5d, until the surface of the culture medium is covered with cotton-wool white hyphae, and the back of the culture medium appears white stripes, indicating that the hyphae have grown all over the culture medium.

4. Proper temperature difference stimulation is the key to the primordium differentiation. The temperature can be reduced by 5℃ and kept at 20℃, and the light can be seen for 24 hours, and the relative humidity is kept at 70% ~ 80%. After 24 hours, the white mycelium will gradually turn orange, and a large number of primordium will appear in 3 ~ 5 days.

Continuous full illumination, mycelium covering the whole culture medium, primordium differentiation is early and dense, and the density of primordium determines the number of fruiting bodies, which is the premise of stable and high yield of Cordyceps militaris.

5. After the differentiation of the management primordium 30min the constant temperature growth period (grass emergence period), when the grass grows about 2cm, the room temperature can be adjusted to 22℃, and the glass bottle cap can be ventilated with small holes, with light at night 14h and no light during the day 10h, and the relative humidity can be increased by 90_, and the ventilation can be continued for about 20 days.

6. Timely harvesting is the key to high yield. The growth period of Cordyceps militaris is 40 ~ 50 days. Early harvesting will reduce the yield, while late harvesting will affect the commodity value of fruiting bodies.

When the fruiting body grows to 8 ~10 cm, and the head expands, it is harvested before spore powder is emitted, dried for one day, and stored in an air-conditioned room at a constant temperature without light.

The key to the difficulty of stable cultivation of Cordyceps militaris lies in the rapid degradation of strains, so taking key technical measures to maintain the vitality of strains will reduce unnecessary losses and achieve the goal of high yield.

Note: Cordyceps militaris contains some nutrients similar to Cordyceps militaris, and its price is relatively low, and its artificial cultivation technology is basically mature, which has been well promoted.