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Operation procedure of agarose gel electrophoresis

Prepare clean gel plates and electrophoresis tanks

Note that instruments contaminated with DNA enzymes may degrade DNA, resulting in weak, blurred or even missing band signals. Electrophoretic elution method

Low melting point agarose gel electrophoresis scooping method

Freeze-thaw recovery method

Glass milk recovery method

Column recovery method Wash the electrophoresis tank with ddH2O repeatedly and pour in the freshly prepared sterilized electrophoresis buffer;

Preparation of agarose gel plate of appropriate thickness according to the amount of spotting;

Cutting of gel Cut off the gel without DNA fragments as much as possible;

To minimize the exposure time of DNA under UV to reduce the damage to DNA;

Melt the gel completely.