Leaf surface view: the upper and lower epidermal cells are polygonal, and the vertical wall is flat or slightly curved. The upper and lower epidermis are covered by non-glandular hairs, especially the main veins; Single cell without glandular hairs, conical, warty or smooth wall, 50-70μm long, and 20-40μm in base diameter. The pores are mainly flat axes with a few infinitives. Leaf cross section: upper and lower epidermal cells 1 row, nearly rectangular, with flat peripheral wall of epidermis and slight keratinization. The mesophyll tissue is isoplanar, the palisade tissue of the upper epidermis is mostly 2 rows of cells, and the lower epidermis is mostly 1 row of cells, which is short and contains a small amount of calcium oxalate crystals, and the sponge tissue has 2-4 rows of cells. The vascular bundle of the main vein is double-tough, and the laticifer is scattered around the vascular bundle and phloem. The inner side of the upper and lower epidermis of the main vein is a series of thick horny tissues.

thin layer chromatography

Take 0.5g of this product powder, moisten it with 5% hydrochloric acid 1ml, add ethyl acetate 15ml, put it in water, heat and reflux 1h, filter, evaporate the filtrate, and add methanol 1ml to dissolve the residue as the test solution, with quercetin methanol solution as the control. Spot samples on the same silica gel G-0.5% CMC thin-layer plate, developed with toluene-chlorine-acetone-formic acid (5: 8: 7: z), and examined under ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots with the same color appear in the corresponding position of the chromatogram of the control sample.