The electrical properties of horseradish peroxidase need to be substituted into the specific pH environment in order to determine.

1, the isoelectric point of horseradish peroxidase is pH 3 to 9, in this pH range, horseradish peroxidase is electrically neutral.

2. If the environmental pH is greater than 9, horseradish peroxidase is electronegative.

3. If the environmental pH is less than 3, horseradish peroxidase is positively charged.