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Medicinal Value of Cistanchiakia

Introduction of medicinal use: Cistanche (cōng, róng), also known as the large rue, has been used by the countries in the western region as a tribute to the imperial court in history.

Cistanchis have been used in medicine for a long time. It is sweet and warm, salty and moist, with the characteristics of tonifying yang without dryness, warming kidney yang and tonifying kidney deficiency; tonifying yin without greasiness, moistening the intestines to cure constipation. It is because of its tonic and gentle nature, it has the name of Cistanche (from).

Li Shizhen, a medicine expert in the Ming Dynasty, discussed about Cistanchis in his Compendium of Materia Medica as follows:

"Name" is also known as "Cistanches" (肉松容), "Cistanches" (黑司命).

Odor: Sweet, slightly warm, non-toxic.

"Indications"

1. Injury from labor, darkness of face due to loss of essence. Use four taels of Cistanches, boil it in water, cut it thinly and grind it finely, and eat it with stewed mutton.

2. Kidney deficiency with white color. With Cistanchis, antler, yam, white poria, etc. into the end, add rice paste made of pills, such as Wuzi large, each serving thirty pills, date soup sent down.

3, sweaty constipation (old or weak patients). With Cistanchis (wine soaked, roasted) two two, sedum end one or two, *** research for the end. Add hemp seed juice paste into pills, such as the size of a stout. Take seven or eight pills each time. The water to send down.

4, eliminate in easy to hunger (according to: suffer from this disease often eat more, but the body is very thin). With Cistanchis, cornelian cherry, schizandra and other points, *** research for the end, add honey into pills, such as a big stout. Each serving twenty pills, salt and wine sent down.

5, tetanus (mouth forbidden, body straight). With Cistanchis sliced and dried, burned into the smoke smoked wound, tired effect.

Pharmacologists about Cistanche and further analyzed and studied the nature and function of Cistanche. One of them wrote: "Da Yun and mutton boiled food, can cure 'five for seven injuries', abdominal cold and heat disease, strong yin and benefit essence", 'with its warm and can moisten, slippery and not diarrhea, often supplement not june, june and essence blood, Xing Yang help people! "It is said that it "nourishes the five organs, strengthens the yin, and benefits the essence"; and it is said in Da Ming that it treats "men who have no yang, and women who have no yin". The medical profession applies modern scientific methods to analyze Cistanchis, and the results show that Cistanchis contains trace alkaloids and crystalline neutral substances, and it does have the efficacy of treating cold pain in the waist and knees, infertility of women, neurasthenia, and hearing loss, and it has the effect of lowering the blood pressure, and it can be used as a hemostatic medicine for cystitis, bleeding of bladder, and bleeding of kidneys.

Origin Huyi Xi Plateau, Ulanqab, Ordos, East Alxa, West Alxa. Gansu, Qinghai, Xinjiang, China; Russia, Mongolia, Iran.

Mongolian Name Hu Ji Ri Seg a Chagan Gao Yao

Alias Cistanche

Scientific Name Cistanche salsa (C. A. Mey.) G. Beck (Ledebouriidae)

Morphological Characteristics Perennial parasitic herb. Stem endomorphic, terete, yellow. Scalelike leaves ovate to torulose-lanceolate, closely spaced on lower part of stem, lax and tapering on upper part, yellow or tawny. Spikes terete, 5-20 cm; bracts ovate or momentarily circular-lanceolate; bracteoles lanceolate-rectangular, margins ± woolly or glabrous, subequal to calyx; calyx campanulate, yellowish or white, ca. 1/3 as long as corolla tube, 5-lobed, lobes ovate or suborbicular; corolla tubular-campanulate, tube white, lobes semiorbicular, lilac, tube with 2 convex lines off-axis. surface off-axis with 2 raised yellow longitudinal stripes, often retaining original color after drying; stamens 4, didynamous, nearly included, anthers and filaments crinkly-villous at base, anthers with apical polytrichous cusps; ovary superior, with 4 "d"-shaped lateral membranous placentas. Capsule ellipsoid, 2-valved; seeds subglobose, 0.4-0.5 mm in diam. Flowering in May-June, mostly after the rain out of the ground, fruiting in June-July.

Origin and production Production Xilin Gol League, the northwestern part of the Ulanqab League, the western part of the Ikezhaomeng League, Bayannur League, the Alxa League, and Gansu Hexi area production is more.

Growth habit Cistanche is a parasitic plant in the desert trees pike, red willow roots, the soil, water requirements are not high, is a more promising industry. Distributed in Inner Mongolia, Ningxia, Gansu and Xinjiang, it is known as "desert ginseng" and has high medicinal value. Cistanchis are the fleshy stems of Cistanchis or Cistanche and Cistanches in the family of Ledebouriae. It can tonify the kidney, benefit the essence, moisten the dryness, and smooth the intestines, and is used for treating impotence in men, infertility in women, hypogonadism, hematochezia, cold pain in the waist and knees, and constipation withered by blood.

Medicinal Part The fleshy stem is used as medicine.

Collecting and processing In the spring, remove the Chinese order, wash the soil, dry in the sun, sliced and spare.

Taste and Function Chinese medicine is sweet, salty and warm in nature. It is used to tonify the kidney, strengthen the yang, benefit the essence and moisten the intestines.

Mongolian medicine is sweet, salty and warm in nature. It calms "xiezhi", eliminates food, and benefits the essence.

Main Treatment Chinese medicine treats internal injuries from deficiency labor, cold pain in the waist and knees, impotence, slippery semen, infertility, and constipation in a weak body.

Mongolian medicine for acidity, stomach distension, "Xiezhi" headache, impotence, spermatorrhea, premature ejaculation, red and white belt, waist and leg pain.

Dosage and Administration Chinese medicine 6-15 grams, decoction with water, or into pills and proteins.

Mongolian medicines are mostly used in pills and bulk preparations. 1, the effect on the immune function of mice Cistanche aqueous solution of 50mg/kg or 100mg/kg was given to mice to observe the immune function indicators. Results: the weight of spleen and thymus increased from 85±12 and 37±6mg/kg to 140±12 and 53±6mg/kg; the phagocytosis rate of macrophage increased from 53±5% to 78±3%; the values of hemolysin and hemolysed vacuoles increased from 147±47 and 0.05±0.1 to 361±62 and 0.18±0.01, respectively; the content of CAMP in the peritoneal macrophages increased from 100±8.6pm to 0.18±0.01, respectively. 100±8.6 pmol/ml to 152±10.9, and cGMP content decreased from 62±12 (pmol/ml) to 39±7; increased lymphocyte transformation rate, which increased the amount of 3H a TdR-involved lymphocytes from 178±19 (cpm) to 589±139; and increased the delayed hypersensitivity reaction from 0.54±0.15 (mm) to force 0.82±0.12. It showed that the water-soluble component of the product enhanced both humoral and cellular immunity in mice.

2, on human lymphocytes E knot formation and acid a-acetate naphtholase esterase (ANAE) activity: normal human peripheral blood lymphocytes and the drug in vitro at 37 ℃ **** temperature for 1 hour after the determination of active E (Ea) and total E (Et) knot rate. Flower knot smears were stained for ANAE. The results showed that the positive control drugs porcine thymosin F5 (500ug/ml), levamisole (10ug/ml) and Astragalus at low concentration (5mg/ml), and the kidney tonic Cistanche at low concentration (5mg/ml) increased the Ea nodule rate, but had no effect on the Et nodule rate. And both of Cistanche at high concentration (50mg/ml) decreased the Et flower knotting rate. Porcine thymosin F5 and levamisole did not increase the percentage of ANAE+ lymphocytes, but Cistanthus could decrease the percentage of ANAE+ lymphocytes at high or low concentrations. It can be seen that the kidney tonic Cistanche can promote the formation of Ea flower knot at a certain concentration, and the E flower knot test is a commonly used method to determine the number of human T cells, which to a certain extent can reflect the cellular immune function of the organism.3. The effect of Cistanche on the delayed footpad reaction in mice: mice ig herbal decoction (60%, 0.5 ml) for 8 consecutive days, administration of dipSRBC attack on dipSRBC, and measurement of footpad swelling 24 hours later Values. The results showed that the relative intensity of foot pad response in the Cistanche group was higher than that in the control group at 24 hours after the attack, and that Cistanche has the effect of promoting cellular immune function in vivo.

4. Effects on the digestive system:

(1) Promoting defecation: All groups of Cistanchis can significantly shorten the time of defecation in mice, which has the effect of promoting defecation. The shape of the feces was observed at the same time, and the fecal pellets in each group were normal or slightly larger than those in the other groups, while individual mice (5%) had diluted watery feces.

(2) Effects on the propulsive function of small intestine in mice: female mice weighing 33±39, the drug solution and the control solution of distilled water were mixed with charcoal powder (the dosage was 5% of the drug solution) before administration. Mice were fasted on water supply for 16 hrs at 0.6 ml/only ig each test solution and executed after 20 min. The small intestine was removed by dissection and measured. The pylorus to the furthest point of the end of the charcoal traveled as the degree of propulsion, the pylorus to the ileocecal valve as the full length, and the former divided by the latter as the degree of propulsion. All groups of Cistanche were able to significantly increase the degree of propulsion of small intestine in mice, which proved that the drug could enhance intestinal peristalsis and had the effect of improving the function of intestinal muscle motility.4.3. Cistanche's antagonistic effect on the inhibition of defecation by atropine: Male mice weighing 42±39 (8mo) and female mice weighing 46±4g (8mo) were taken and randomly divided into 4 groups according to their body weights, with half of the mice and half of the women and half of the men. After 16 hours of water fasting and water supply, the water-only group was given ig each test solution at 0.15 ml/10 g body weight. The time of ip atropine, the time of ig each test solution and the time of mice discharging the first red feces were recorded to calculate the laxative time. The results showed that 0.025% atropine had a significant inhibitory effect on defecation in mice, and Cistanche effectively counteracted this inhibitory effect of atropine, and its counteracting strength was not significantly different from that of Gastrofen (P>0.47).

(3) The effect of Cistanche on water absorption in the large and small intestines of mice: 20 male mice (8mo) weighing 43±3g and 20 female mice (8mo) weighing 43±3g were selected and randomly divided into 4 groups according to their body weight. After 16 hours of fasting water supply the control group was ig distilled water at 0.15ml/10g body weight and the Cistanche group ig equal amount of 50% Cistanche original herb decoction. Half of the mice were executed 3 hours and 5 hours after drug administration, dissected, and the ends of large and small intestines were clamped with hemostatic clips, and the large and small intestines were separated and cut off, respectively, and placed on weighing paper, then the hemostatic clips were loosened, and the wet weight was weighed on a balance immediately. After drying in the oven at 105°C for 2 hours, the dry weights of the large and small intestines were weighed. The results in Table 4 showed that after 5 hours of administration, the difference in the water content of the large intestine in the Cistanche group was very significant compared with that of the control group, suggesting that Cistanche had an inhibitory effect on the water absorption of the large intestine.

5. Pharmacological effects of the concocted products: Cistanche made by different concoctions (see chemical composition bis for concoctions) was given to mice at a dose of 0.5g/only for ***10 days, and compared with the synthesis rate of deoxyribonucleic acid (DNA) of liver and spleen of Yangxu (a model made of hydrocortisone acetate) mice. The results showed a decrease in the DNA synthesis rate in the Yang deficiency group and an increase in the DNA synthesis rate in the administered treatment group, with P values of <0.05 and <0.01, etc., respectively. Drug properties

It is flat cylindrical, flat or curved, 4-15 centimeters long, 1.5-2.5 centimeters in diameter. The surface is reddish brown to brownish brown, densely covered with fleshy scale leaves arranged in a tile-like manner, the apex of which is often mutilated. Weight, slightly hard and slightly tough, easy to break. Cross-section uneven, dark brown, gray-white vascular bundles strongly curved into ring-like corrugation. Gas faint, taste sweet, into and slightly bitter.

Identification stem cross-section

Epidermal cells class rectangular, externally covered with cuticle. The cortex is slightly broad and thick, and there are several 10 layers of irregular pigmented cells on the outer side, containing yellowish brown material, and the inner thin-walled cells contain starch granules. Endodermis inconspicuous. The mesostyle vascular bundles are strongly undulate arranged in rings, each bundle is rhombic, and the pith is often fissured or hollow.

Powder characteristics

Brownish-brown. (1)Pore pattern cells are oblong, orbicular-like, slightly thick-walled, slightly lignified, with obvious pore grooves, and with fissure-like unilocular pores. (2)The conduit is much broken, mainly threaded and reticulated, the intact one is 130-300 micrometers long and 10-20 micrometers in diameter; the ladder and pore conduit is visible, 60-130 micrometers long and l0-30 micrometers in diameter. (3) Fibers scattered, long pike-shaped, 40 a 200 microns long, 6-20 microns in diameter, thin wall, pore grooves are obvious, the cellular cavity is larger, single grain pore cleavage-like. (4) Starch grains are rare, single spherical, 3-20 a 48 microns in diameter, umbilical point mostly in the middle or small end, fissure-like, punctate or cruciform, laminae obvious. (5) Scalar leaf fragments are visible, section isohedral type.

Physical and chemical identification

1. Take 0.5g of the powder, add 5ml of 70% ethanol, warm it in water bath for 10min, and filter it. The filtrate was evaporated, plus glacial acetic acid 1ml poured into a test tube, along the wall of the tube with sulfuric acid 1ml, the interface of the two liquids have a brown-red ring. (Check steroid) (2) Take 0.5g of the powder, add 5% hydrochloric acid solution 5ml, warm up in water bath for 20min, filtered. The filtrate was added with bismuth potassium iodide reagent to produce a brownish red precipitate. (Check alkaloids) (3) Thin-layer chromatography Take 1g of the powder of the product, add 10ml of ethanol and macerate for 2h, filtered. The filtrate was evaporated and dissolved with 1ml ethanol as test solution. Another take mannitol, add ethanol to dissolve into a solution containing 1mg per milliliter, as a control solution. Take 10μm of each of the above two solutions, were spotted on the same silica gel H plate, with n-butanol - glacial acetic acid ethanol - water (4:1:1:2) unfolding, spreading distance of 12cm, take out to dry, spray 10% cerium ammonium nitrate ethanol solution to develop color. The chromatogram of the test solution and the chromatogram of the control product have the same brown-yellow spots on the corresponding position.

Chinese medicine heritage: drug source

Shen Nong Ben Cao Jing, Volume 1: "Cistanches: sweet and slightly warm flavor, the main five labors and seven injuries, tonifying the middle, in addition to the stem of the cold and heat pain, nourishing the five viscera, strengthening the yin, benefiting the vital essence, many children, the woman's obstruction in the abdomen, take a long time to lighten the body." Sanguo Wei Wu Pu Note: "Cistanches, one of them is Cistanches. Cistanchiakia, a meat pine hibiscus." 1. Ben Jing: Mainly used for five labors and seven injuries, tonifying the middle, removing cold and heat pain in the stem, nourishing the five viscera, strengthening the yin, benefiting the vital essence, and treating obstruction in the abdomen in women.

2. "Bielu": removing evil spirits from the bladder, lumbar pain, and stopping dysentery.

3. "Treatise on the Nature of Medicine": benefit the marrow, pleasing to the color, prolonging life, treating women's hematochezia, strengthening the yang, a great tonic, the main red and white down.

4. "Rihuazi Materia Medica": treatment of male absolute yang does not rise, female absolute yin does not give birth, moistening the five viscera, long muscles, warming the waist and knees, men's ejaculation, blood in the urine, leeching, with the yin pain.

5. "Materia Medica Jingshu": white wine boiled and eaten, treating the elderly dry and closed.

6. "Classic of the Materia Medica": Cistanchis, nourishing the kidney and tonifying the essence and blood of the key medicines, the gas is slightly warm, and the rumor is that it is a mistake to think that it is hot. Gan can remove heat and replenish the middle, acid can enter the liver, salty can nourish the kidney, kidney and liver are yin, yin qi nourishment, then the labor of the five organs of the heat from the penis, cold and heat pain from the penis. If the kidney and liver are sufficient, then the essence and blood will be abundant, and if the essence and blood are abundant, then there will be more children. The obstruction in a woman's abdomen is in the blood; if the blood is abundant, the obstruction will go away. The bladder is weak, then the evil guest, get tonic is the evil dispersed, back pain since the end. Long-term service is fat and healthy and light body, benefit the kidney and liver to replenish the effect of essence and blood, if said to cure dysentery, not slippery to guide stagnation of the meaning of the word, this also must not be said.

7. "Materia Medica": Cistanchis, support the life, nourish the kidney gas, replenish essence and blood of the drug also. Men's Danyuan is cold and sunny for a long time, and women's Yin Qi is not cured by the disorder of Chong Ren, this is a flat tonic agent, warm but not hot, tonic but not harsh, warm but not dry, slippery but not leakage, so it has the name of calmness.

8. "Ben Jing Feng Yuan": Cistanches, "Ben Jing" main labor injuries in the middle of the tonic, is the fire can not be born in the earth, not the original deficiency of the central qi also. It is also used in the treatment of obstruction in the abdomen of women, as it can soften the hardness and take away the blood. Cistanchis can stop spermatozoa, remove heat and pain in the stem, because it can guide the fire down. The elderly dry knot, it is appropriate to cook porridge to eat.

9. Jade Rowan Pharmacopoeia: Cistanchia, warming the waist and knees, strengthening the bones and flesh, nourishing the kidney, liver, essence and blood, and moistening the dryness of the stomach and intestines. Where fecal pellets are firm and small, shaped like sheep's feces, this is the reason why the earth is wet and the wood is depressed and the lower orifices are blocked. The dregs of grain in the stomach, not down, sporadic transmission, disconnected, the dryness of the yangming large intestine, refining into particles, secret and difficult to pass, the total edge of the wind and wood withered, sparing not also. A service of dihuang, turtle gelatin, anti-gain soil moisture, the more defeated in the gas carry on. Cistanches nourish wood clear wind, nourish blood and moisten dryness, good slippery colon, and under the knot of feces, the nature of the calm and unhurried, not to nourish the wet defeat of the spleen, non-runner comparable to the drug. Fang Shu said that it replenishes the marrow, pleases the color and prolongs the life, the rationale for men's extinct yang not to rise, women's extinct yin not to give birth to, not a complimentary word. 10. "The Materia Medica Seeking the Truth": Cistanche, all the books said that it is a harsh tonic for the vital essence and blood, and said that it can raise the yang to help the fire, it is because of its temperature, the force of the special nourishing yin, and the yang with yin attached to the yang, and the yang from the see Xing ear. However, its power can nourish and tonify, so all the obstructions and lumps in the abdomen will be eliminated if they are nourished by it. But its nourishing and yang to help, so all spermatorrhea stem pain, cold and fever, but also because of the removal. If it is said that the fire to the extreme, the use of this sweet and moist products, the same as gui, attached to the force can be complementary to the sun, the loss is far away. Besides, it is also said to nourish yin, and nourish yin with the name of Cistanche, it is because its power is not sudden, and the gas is specialized in moistening and drying, it is also suitable for the people with closed stools and not suitable for the people with stomach deficiency. It can be called nourishing Yin, but it is not necessarily called tonifying fire.

10. "Justice of the Materia Medica": Cistanches, the main treatment of the "Benjing", all to hide the yin, the main labor injuries to fill the middle, nourish the five viscera, strong yin, are all the yin tonic function. Cold, heat and pain in the stem is a disease of kidney coldness. Cistanchu is thick and heavy and descends directly into the kidney family, warm and moist, without the harm of dryness and intensity, and can warm and nourish the essence and blood and pass the yang qi, so it is said to be beneficial to the essence and qi. It can warm and nourish essence and blood and pass Yang Qi, so it is said to be beneficial to essence and blood. The main obstruction in the abdomen is that it can soften the hardness and enter into the blood, and it can nourish yin essence and nourish Yang Qi, which makes qi and blood fluent and passes through. The "Beilu" in addition to the bladder evil, but also warm and nourish the water and cold since the elimination of the evil. The waist of the kidney, kidney deficiency is lumbago, Cistanchu benefit kidney, is to cure. Li, this wood is all for dysentery, is stagnant and not fast stagnation, non-diarrhea of self-liberty, Cistanche slippery bowel, dysentery for stagnation, it is desirable to dredge and should not be fixed astringent, slippery to go to its own, but also can raise the five viscera and is not dedicated to attack and chase, for a long time dysentery in the gas of their own virtual, and stagnation is not exhausted the desirability of the non-comprehensive treatment of summer heat and dampness and heat stagnation dysentery is also. Cistanchis are extremely moist products, the market are salted, is able to hide for a long time, the ancient books are said to be slightly warm, and now it is salty for a long time, the warmth has been removed from the net, even if the rinse is very light, and the nature of the elimination of more, so the ancients said to complement the yin Xing Yang all kinds of efficacy, all very weak, cover has been used with the club, no longer

Can be the quality of the original example of theory carry on. But the salty flavor can fall, slippery can pass the intestine, to the main bowel irregularities, quite a shortcut effect, and the nature of the warmth, yin through the yang, so through the internal organs and does not hurt the fluid, especially the unique ear. Since the Song Dynasty, Cistanchiakia is the main ejaculation and even the main hematochezia and drowning of blood, which is used to replenish the yin and help the yang, and it is said that it has the effect of taking in and fixing the yin. To know the slippery products, through the conductive surplus, Xi can be fixed astringent, "this scripture" in addition to the yin in the cold and hot pain, is to tonify the yin through the yang, through the pain is not ear. It is the later derivation of its meaning, mistakenly recognized as a big complement, and want to Tongli treatment of slippery, fallacy. Weak stomach and loose stools, phase of fire is not to be served.

1. "Materia Medica Monchuan": avoid the iron.

2. "Materia Medica Jingshu": diarrhea is prohibited, there is heat in the kidneys, the strong yang is easy to rise and the essence of the people who are not solid.

3. "Drugs": phase fire, weak stomach and intestines are contraindicated.

4. "Getting and Matching Materia Medica": avoid copper and iron. It is also contraindicated in cases of fire, closed stool, and heart deficiency and gas distension. 1. Jichuan Decoction ("Jingyue Quanshu - New Formulas and Eight Formulas") is used for treating kidney deficiency, weak qi, inability to pass stools, long and clear urination, and lumbar soreness and back coldness.

2. Dihuang Drink (Emperor Suwen Xuanming Lunfang), nourishing kidney yin, tonifying kidney yang, opening up the orifices and resolving phlegm, treating outpatient disease, with a strong tongue that is unable to speak, a foot that is unable to be used, and a dry mouth that does not want to drink.

3. Unparalleled Yam Pill ("备急千金要方"), strengthens the spleen and stomach, nourishes the kidneys, treats deficiency labor injury, emaciation, lumbar soreness and knee weakness, darkness of the eyes and tinnitus, and tastelessness of food.

4. Jiawei Sijinwan ("Three Causes of Extreme Disease") is used for treating deficiency of the liver and kidney, heat in the body, impotence and weakness of the muscles and bones, weakness in walking, lumbar and knee soreness and weakness, hot flashes, and tastelessness in food and drink.

5. Cuscuta Zi Pill ("Taiping Huimin Hejiao Bian Fang"), the main treatment of kidney qi deficiency, five labors and seven injuries, abdominal constriction, shortness of breath in the limbs, nightmares and panic, sleepiness, temperamental, urination, room inactivity, and so on.

6. Cuscuta chinensis (菟丝子散) (《医宗必读》), is used for treating bladder deficiency and cold, incontinence of urination or excessive urination.

7. Cistanches Pill ("Medical Heart Formula"), treating men with five labors and seven injuries, impotence, dribbling of urine, and reddish-yellow urine.

8. Ginseng and antler pills ("National Collection of Traditional Chinese Medicines"), treating physical weakness and fatigue, tinnitus and palpitation, spermatorrhea and premature ejaculation, anemia and atrophy, neurasthenia.

9. Run intestines pill ("Shiji doctor to get effective prescription"), the main treatment of fluid deficiency, constipation. 1. Cistanchis: clean impurities, soaked in water, water 1-2 times a day (such as the Department of salty Cistanchis, soak all the salt), moistened, sliced (longitudinal cut), sun-dried.

2. Wine Cistanchis: Take the slices of Cistanchis, mix them with yellow wine, put them in a jar, sit in a pot of water, heat and steam the water until the wine is finished, take them out and dry them. (Each 100 kg of Cistanchia, 30 kg of yellow wine)

3. Black bean system: take Cistanchia and soak it in rice swill water for 3 days, change the water once a day, remove all the salty taste, scrape off the surface scale leaves, cut 1.5 cm thick slices; then take 5 kg of black beans and fry them, divide them into 3 parts, each time take 1 part of them and mix them with water and Cistanchia and dry them with light fire, take them out and make them half-dried, then dry them by steaming them and then dry them, and then take another part of them to cook, steam, dry them by repeating the procedure 3 times, and then dry them. It can be dried in the sun for 3 times. For every 500kg of Cistanche, 50kg of black beans are used.

4. "Lei Gong Gun Zhi Lun": Wherever Cistanche is used, it should be soaked in wine for one night, and when it comes to the morning, it should be brushed with brown brush to get rid of sandy soil and floating armor, and it should be split in the center, and it should be removed to the white membrane, such as a heavy bamboo grass-like one, and it should be steamed, and then it should be taken out from noon to you and then it should be roasted with crisps.