Contents 1 Pinyin 2 English reference 3 Overview 4 Medical examination of urinary amylase 4.1 Test name 4.2 Classification 4.3 Laboratory materials 4.4 Determination principle of urinary amylase 4.5 Reagents 4.6 Operation method 4.7 Normal value 4.8 Clinical significance of laboratory results 4.9 Note 4.10 Related diseases 1 Pinyin

niào diàn fěn méi 2 English reference

Urine amylase 3 Overview

Under normal circumstances, there can be a small amount of amylase Absorbed into blood and excreted in urine. Measuring urinary amylase can help diagnose pancreatic diseases. 4 Medical examination of urinary amylase 4.1 Test name

Urinary amylase 4.2 Classification

Body fluid and excreta examination> Urine examination

4.3 Laboratory materials

Urine 4.4 Principle of determination of urinary amylase

(1) Iodine starch colorimetric method: The starch in the matrix is ??hydrolyzed by α-amylase in the specimen to generate glucose, maltose and dextrin. The remaining starch combines with the iodine solution to form a blue complex, the depth of which is inversely proportional to the enzyme activity.

(2) p-Nitrophenyl maltoheptaose method: p-nitrophenyl maltoheptaose under the action of AMS, generates nitrophenyl maltotriose, p-nitrophenyl maltototetraose, and maltotriose and maltotetraose. The former continues to be hydrolyzed into p-nitrophenol and glucose under the action of α-glucosidase, and the production rate of p-nitrophenol is proportional to AMS activity. 4.5 Reagents

(1) Iodine starch colorimetric method:

①Matrix buffer (0.4g/L starch): Weigh NaCl 9g, Na2HPO4 22.6g (Na2HPO4·12H2O 56.94 g) and 12.5g of KH2PO4, dissolve in about 500ml of distilled water, and heat to boiling. Take another small beaker, accurately weigh 0.4g of soluble starch, add about 10ml of water, suspend it, add it to the above boiling solution, rinse the beaker with water and pour it together. After cooling to room temperature, add 5ml of 37% formaldehyde solution and dilute to 1000ml with distilled water. The pH is 7.0±0.1 and should be stored in the refrigerator.

② Iodine storage solution (0.1mol/L): Weigh 1.7835g of potassium iodate and 22.5g of potassium iodide and dissolve it in 400ml of water. Slowly add 4.5 ml of concentrated hydrochloric acid, stir while adding, dilute to 500 ml with distilled water, mix well, place in a brown bottle, and store in the refrigerator.

③ Iodine application solution (0.01mol/L): Take the iodine storage solution, dilute it 10 times with distilled water, store it in a brown bottle, and store it in the refrigerator for 1 month.

(2) p-Nitrophenyl maltoheptaose method: the main ingredients are: 3000U/L α-glucosidase, 5mmol/L p-nitrophenyl maltoheptaose, 100mmol/L phosphate buffer ,50mmol/L NaCl.

The commercial kit is reconstituted according to its instructions and can be stored in the refrigerator for 3 days. 4.6 Operation method

(1) Iodine starch colorimetric method: Serum is diluted 10 times with physiological saline, urine is diluted 20 times, and the matrix buffer is prewarmed at 37°C for 5 minutes. Operate according to Table 1.

Mix well, use 660nm wavelength, 1cm light path, adjust zero point with distilled water, and read the absorbance of each tube.

(2) p-Nitrophenyl maltoheptaose method: It can be programmed according to the laboratory analyzer model and instructions. The main parameters are: wavelength 405nm, 37°C, and the ratio of specimen to reagent volume is 1: 20 (the urine is diluted 1 times with physiological saline before the experiment, and the result is multiplied by 2), delay time 3min, linear time 10min, kinetic method. 4.7 Normal value

(1) Iodine starch colorimetric method: 1000~12000U/L;

(2) p-Nitrophenyl maltoheptaose method: 120~1200U/L.

4.8 Clinical significance of laboratory results

Reduce: severe hepatitis, cirrhosis, diabetes, severe burns, etc.

Elevated: seen in acute pancreatitis and pancreatic duct obstruction caused by any reason (such as pancreatic cancer, pancreatic injury), acute cholecystitis, perforated gastric ulcer, alcoholism, mumps, etc. 4.9 Notes

Oral contraceptives, sulfonamides, thiazide diuretics, codeine, morphine, steroids, analgesics, and sample contamination by saliva can cause high test results. Anticoagulants such as oxalate, citrate, and ethylenediaminetetraacetic acid can inhibit the activity of AMS, resulting in low measurement results. 4.10 Related diseases