1. Manually separate the target amplification band by using tools such as gel cutter or gel pad, and introduce it into PCR tube for further analysis, such as DNA purification or sequencing.

2. Using polyacrylamide gel instead of agarose gel for electrophoresis, the resolution of polyacrylamide gel is higher and DNA fragments can be separated better. After multiple bands are detected, the target bands are extracted by cutting them in the gel.